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An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting
Nanoscale ( IF 6.7 ) Pub Date : 2022-11-18 , DOI: 10.1039/d2nr05269e
Tadija Kekić 1 , Jory Lietard 1
Affiliation  

The ability to regulate, maintain and reproduce fluorogenic properties is a fundamental prerequisite of modern molecular diagnostics, nanotechnology and bioimaging. The sequence-dependence of the fluorescence properties in fluorophores commonly used in nucleic acid labelling is here being exploited to assemble a color scale in 256 shades of green Cy3 fluorescence. Using photolithography, we synthesize microarrays of labeled nucleic acids that can accurately reproduce 8-bit monochrome graphics by mapping color to fluorescence intensity and sequence. This DNA-based painting approach paves the way for a full RGB scale array fabrication process.

中文翻译:

用于基于 DNA 绘画的荧光核酸序列的 8 位单色调色板

调节、维持和再现荧光特性的能力是现代分子诊断、纳米技术和生物成像的基本先决条件。核酸标记中常用的荧光团的荧光特性的序列依赖性在这里被利用来组装 256 种绿色 Cy3 荧光色调的色标。使用光刻技术,我们合成了标记核酸的微阵列,通过将颜色映射到荧光强度和序列,可以准确地再现 8 位单色图形。这种基于 DNA 的绘画方法为完整的 RGB 比例阵列制造过程铺平了道路。
更新日期:2022-11-23
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