Artificial intervention combined with stress acclimation was used to screen a heterotrophic nitrifying-aerobic denitrifying (HN-AD) bacterial, strain Rhodococcus SY24, resistant to linear alkylbenzenesulfonic acid (LAS) stress. When LAS was<15 mg/L, strain SY24 performed better cell growth and carbon source metabolism activity. The maximum nitrification and denitrification rates of SY24 under LAS stress could reach 1.18 mg/L/h and 1.05 mg/L/h, respectively, which were 13.80 % and 8.81 % higher than those of the original strain CPZ24. Higher LAS tolerance was seen in the functional genes (amoA, nxrA, napA, narG, nirK, nirS, norB, and nosZ). Response surface modeling revealed that 2 mg/L LAS, sodium succinate as a carbon source, 190 rams, and carbon/nitrogen 11 were the ideal culture conditions for SY24 to nitrogen removal under the LAS environment. This study offered a new screening strategy for the functional species, and strain SY24 showed significant LAS tolerance and HN-AD potential.

人工干预结合胁迫驯化筛选出一株耐直链烷基苯磺酸 (LAS) 胁迫的异养硝化-好氧反硝化 (HN-AD) 菌株Rhodococcus SY24。当LAS<15 mg/L时，菌株SY24表现出较好的细胞生长和碳源代谢活性。LAS胁迫下SY24的最大硝化和反硝化速率分别达到1.18 mg/L/h和1.05 mg/L/h，分别比原菌株CPZ24提高了13.80 %和8.81 %。在功能基因（amoA、nxrA、napA、narG、nirK、nirS、norB和nosZ ）中观察到更高的 LAS 耐受性). 响应面模型显示，2 mg/L LAS、琥珀酸钠作为碳源、190 rams、碳/氮 11 是 SY24 在 LAS 环境下脱氮的理想培养条件。该研究为功能菌种提供了一种新的筛选策略，菌株 SY24 显示出显着的 LAS 耐受性和 HN-AD 潜力。