Abstract

Three new proaporphine alkaloids, cissamaline (4), cissamanine (5), and cissamdine (6), along with two known compounds (2 and 3) were isolated from total tertiary alakloidal extracts of the leaves and stems of Cissampelos capensis. The new alkaloids were elucidated through comprehensive spectroscopic analysis, including 1 D, 2 D NMR and High-resolution LCMS. The in vitro MTT cytotoxicity was evaluated on Caco-2 cell lines, where all the isolated compounds exhibited cytotoxic effects at concentrations above 250 µM.

中文翻译：

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Cissampelos capensis Lf 中三种新的阿朴啡原生物碱及其细胞毒性评价

摘要

从Cissampelos capensis叶和茎的总三级生物碱提取物中分离出三种新的原阿朴啡生物碱：cissamaline ( 4 )、cissamine ( 5 ) 和 cisamdine ( 6 )，以及两种已知化合物（2和3 ） 。通过综合光谱分析（包括 1 D、2 D NMR 和高分辨率 LCMS）阐明了新的生物碱。在 Caco-2 细胞系上评估了体外MTT 细胞毒性，其中所有分离的化合物在浓度高于 250 µM 时均表现出细胞毒性作用。