Abstract

Objectives: An ethanol extract of the whole plants of Saussurea medusa had been investigated to find novel anti-inflammatory sesquiterpenoids. Methods: Extensive spectroscopic data and chemical methods were applied to elucidate the structures of the compounds. Results: One new megastigmane sesquiterpenoid (1), along with 11 known analogues (2–12), were obtained from S. medusa. All isolates, except compounds 3 and 6, were mentioned from the studied plant for the first time. Compounds 1, 2, 4, 5, 7, 8 and 12 were firstly isolated from the genus Saussurea. Compounds 2, 9 and 10 were found to inhibit the lipopolysaccharide (LPS)-induced release of NO by RAW264.7 cells with IC₅₀ values ranging from 21.1 ± 1.7 to 46.7 ± 1.9 μM. Furthermore, iNOS expression experiment was performed to examine the interactions between the active compounds and the iNOS enzyme.

摘要

目的：对水母雪莲全草的乙醇提取物进行研究，以寻找新型抗炎倍半萜类化合物。方法：应用广泛的光谱数据和化学方法来阐明化合物的结构。结果：从水母中获得了一种新的大豆豆倍半萜类化合物 ( 1 ) 以及 11 种已知类似物 ( 2 – 12 ) 。除化合物3和6外，所有分离株均首次从所研究的植物中提及。化合物1、2、4、5、7、8和12是首次从雪莲属植物中分离得到。_ _ _ 发现化合物2、9和10可抑制脂多糖 (LPS) 诱导的 RAW264.7 细胞释放 NO，IC ₅₀值范围为 21.1 ± 1.7 至 46.7 ± 1.9  μM。此外，进行 iNOS 表达实验以检验活性化合物和 iNOS 酶之间的相互作用。