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Comprehensive two-dimensional countercurrent chromatography × gas chromatography characterization of Artemisia argyi essential oil
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2022-11-13 , DOI: 10.1016/j.aca.2022.340614
Weiyi Wen 1 , Ping Xu 1 , Haiping Xiang 1 , Mengyi Wen 1 , Xuemin Ye 2 , Chu Chu 1 , Shengqiang Tong 1
Affiliation  

A comprehensive two-dimensional (2D) countercurrent chromatography (CCC) × gas chromatography (GC) was investigated for characterization of chemical constituents of Artemisia argyi essential oil, and orthogonality for the 2D chromatographic system was evaluated. A solvent system composed of n-hexane/acetonitrile/methanol (2:2:1, v/v/v) was selected for first dimensional separation of Artemisia argyi essential oil. Then all CCC fractions were analyzed by GC, which provided a wealth of information regarding the composition of the essential oil. Visualization of chemical compositions obtained from the comprehensive 2D CCC × GC separation was achieved by creation of a 2D contour plot map. Total peak capacity was evaluated and approximately 1392 peaks were obtained through a comprehensive 2D CCC × GC separation. A high spatial coverage and a low linear correlation coefficient were achieved. Meanwhile, all compounds were identified by GC-MS. The obtained 2D contour plot could be divided into six zones to show the characteristic chemical compositions. Six zones could be divided into different component groups, including monoterpenes, sesquiterpenes, monoterpene alcohols, phenols, aldehydes, ketones and esters, which could be used to identify compounds that have not been reported, and to predict the structure of unknown compounds in Artemisia argyi essential oil and comprehensively characterize fingerprint peak.



中文翻译:

艾蒿精油二维逆流色谱×气相色谱综合表征

研究了综合二维 (2D) 逆流色谱 (CCC) × 气相色谱 (GC) 以表征艾叶精油的化学成分,并评估了二维色谱系统的正交性。选用正己烷/乙腈/甲醇(2:2:1, v/v/v)组成的溶剂体系对艾蒿进行一维分离香精油。然后通过 GC 分析所有 CCC 馏分,这提供了有关精油成分的丰富信息。通过创建 2D 等高线图,实现了从综合 2D CCC × GC 分离中获得的化学成分的可视化。评估了总峰容量,通过全面的 2D CCC × GC 分离获得了大约 1392 个峰。实现了高空间覆盖和低线性相关系数。同时,所有化合物均通过 GC-MS 鉴定。获得的二维等高线图可以分为六个区域以显示特征化学成分。六个区可分为不同的组分组,包括单萜类、倍半萜类、单萜醇类、酚类、醛类、酮类和酯类,艾蒿挥发油及指纹峰综合表征。

更新日期:2022-11-17
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