Poly(3,4-ethylenedioxythiophene) (PEDOT) has been regarded as one of the most attractive conductive materials for making bioelectronics. Surface modification is one of the most important methods to enhance the performance of PEDOT-based bioelectronics. To investigate the surface charge effect on the behavior of protein binding on PEDOT, we develop a new positively charged functionalized EDOT monomer, EDOT-N⁺, to copolymerize with another negatively charged carboxylic acid-modified EDOT monomer, EDOT-COOH. The poly(EDOT-N⁺-co-EDOT-COOH) film is prepared by the electropolymerization method. By mixing the two monomers with different feed ratios, we can tune the distribution of surface charges. The actual composition of the copolymer is determined by toluidine blue O staining. Based on the result of TBO staining, a copolymer with a 1:1 ratio of EDOT-N⁺ to EDOT-COOH is obtained when the monomer solution of composition at [EDOT-N⁺]: [EDOT-COOH] = 8 : 2, which is due to a slower polymerization rate of EDOT-N⁺ compared to EDOT-COOH. To further testify the antifouling property of poly(EDOT-N⁺-co-EDOT-COOH), nonspecific protein binding of bovine serum albumin and lysozyme were measured in situ by using a quartz crystal microbalance. The results also show that at [EDOT-N⁺]: [EDOT-COOH] = 8 : 2, poly(EDOT-N⁺-co-EDOT-COOH) presented good antifouling property. Therefore, we propose a zwitterion-like antifouling property from the poly(EDOT-N⁺-co-EDOT-COOH) with an equal density of both positive and negative functional groups.

聚 (3,4-乙烯二氧噻吩) (PEDOT) 已被认为是制造生物电子学最具吸引力的导电材料之一。表面改性是提高基于 PEDOT 的生物电子学性能的最重要方法之一。为了研究表面电荷对蛋白质在 PEDOT 上的结合行为的影响，我们开发了一种新的带正电荷的官能化 EDOT 单体 EDOT-N ⁺，以与另一种带负电荷的羧酸改性的 EDOT 单体 EDOT-COOH 共聚。聚(EDOT-N ⁺ - co-EDOT-COOH) 薄膜采用电聚合法制备。通过以不同的进料比混合两种单体，我们可以调整表面电荷的分布。共聚物的实际组成通过甲苯胺蓝O染色确定。根据TBO染色结果，当单体溶液组成为[EDOT-N ⁺ ]: [EDOT-COOH] = 8 : 2时，得到EDOT-N ⁺与EDOT-COOH 1:1 的共聚物。 ，这是由于与 EDOT-COOH 相比， EDOT-N ^{+的聚合速率较慢。}进一步验证poly(EDOT-N ⁺ - co )的防污性能-EDOT-COOH)、牛血清白蛋白和溶菌酶的非特异性蛋白结合通过使用石英晶体微量天平原位测量。结果还表明，在[EDOT-N ⁺ ]: [EDOT-COOH] = 8:2 时，poly(EDOT-N ⁺ - co -EDOT-COOH) 表现出良好的防污性能。因此，我们提出了由具有相等密度的正负官能团的聚(EDOT-N ⁺ - co -EDOT-COOH) 提供的两性离子样防污性能。