High-throughput phenotypic cell sorting is critical to the development of cell-based therapies and cell screening discovery platforms. However, current cytometry platforms are limited by throughput, number of fractionated populations that can be isolated, cell viability, and cost. We present an ultrathroughput microfluidic cell sorter capable of processing hundreds of millions of live cells per hour per device based on protein expression. This device, a next-generation microfluidic cell sorter (NG-MICS), combines multiple technologies, including 3D printing, reversible clamp sealing, and superhydrophobic treatments to create a reusable and user-friendly platform ready for deployment. The utility of such a platform is demonstrated through the rapid isolation of mature natural killer cells from peripheral blood mononuclear cells, for use in CAR-NK therapies at clinically-relevant scale.

中文翻译：

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超通量免疫磁性细胞分选平台

高通量表型细胞分选对于基于细胞的疗法和细胞筛选发现平台的开发至关重要。然而，当前的细胞计数平台受到吞吐量、可分离的分级细胞群数量、细胞活力和成本的限制。我们提出了一种超通量微流控细胞分选仪，每台设备每小时能够根据蛋白质表达处理数亿个活细胞。该设备是下一代微流控细胞分选仪 (NG-MICS)，结合了多种技术，包括 3D 打印、可逆夹具密封和超疏水处理，以创建可重复使用且用户友好的平台，可随时部署。这种平台的实用性通过从外周血单核细胞中快速分离成熟的自然杀伤细胞得到证明，