Olive oil has beneficial effects on skin wound healing due to its anti-inflammatory and antioxidant properties; however, the mechanism by which olive oil promotes wound healing is unclear. We evaluated the mechanisms involved in Nrf2 pathway activation by olive oil and its role in cell survival and migration in mouse dermal fibroblasts in a short-term exposition. Our data demonstrated that olive oil and oleic acid promoted reactive oxygen species (ROS) production, while olive oil and hydroxytyrosol stimulated nuclear factor erythroid 2-related factor 2 (Nrf2) activation. Olive oil-mediated ROS production increased nuclear factor kappa B p65 expression, while olive oil-stimulated reactive nitrogen species production augmented the levels of Nrf2. Olive oil augmented cell proliferation, cell migration, and AKT phosphorylation, but decreased apoptotic cell number and cleaved caspase-3 levels. The effect of olive oil on cell migration and protein levels of AKT, BCL-2, and Nrf2 were reversed by an Nrf2 inhibitor. In conclusion, the activation of the Nrf2 pathway by olive oil promotes the survival and migration of dermal fibroblasts that are essential for the resolution of skin wound healing.

中文翻译：

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橄榄油通过Nrf2通路激活促进真皮成纤维细胞的存活和迁移

橄榄油具有抗炎和抗氧化特性，因此对皮肤伤口愈合具有有益作用；然而，橄榄油促进伤口愈合的机制尚不清楚。我们评估了橄榄油激活 Nrf2 通路所涉及的机制及其在小鼠真皮成纤维细胞中的细胞存活和迁移中的作用。我们的数据表明，橄榄油和油酸促进活性氧 (ROS) 的产生，而橄榄油和羟基酪醇刺激核因子红细胞 2 相关因子 2 (Nrf2) 的激活。橄榄油介导的 ROS 产生增加了核因子 kappa B p65 的表达，而橄榄油刺激的活性氮物质产生增加了 Nrf2 的水平。橄榄油增强细胞增殖、细胞迁移和 AKT 磷酸化，但减少了凋亡细胞数量和裂解的 caspase-3 水平。橄榄油对细胞迁移和 AKT、BCL-2 和 Nrf2 蛋白水平的影响被 Nrf2 抑制剂逆转。总之，橄榄油对 Nrf2 通路的激活促进了真皮成纤维细胞的存活和迁移，这对于解决皮肤伤口愈合至关重要。