Neuropathic pain is a debilitating chronic disorder, significantly causing personal and social burdens, in which activated neuroinflammation is one major contributor. Thymic stromal lymphopoietin (TSLP) and interleukin (IL)-33 is important for chronic inflammation. Linalyl acetate (LA) is main component of lavender oil with an anti-inflammatory property through TSLP signaling. The aim of the study is to investigate how LA regulates mechanical hyperalgesia after sciatic nerve injury (SNI). Adult Sprague-Dawley male rats were separated into 3 groups: control group, SNI group and SNI with LA group. LA was administrated intraperitoneally one day before SNI. Pain behavior test was evaluated through calibration forceps testing. Ipsilateral sciatic nerves (SNs), dorsal root ganglions (DRGs) and spinal cord were collected for immunofluorescence staining and Western blotting analyses. SNI rats were more sensitive to hyperalgesia response to mechanical stimulus since operation, which was accompanied by spinal cord glial cells reactions and DRG neuro-glial interaction. LA could relieve the pain sensation, proinflammatory cytokines and decrease the expression of TSLP/TSLPR complex. Also, LA could reduce inflammation through reducing IL-33 signaling. This study is the first to indicate that LA can modulate pain through TSLP/TSLPR and IL-33 signaling after nerve injury.

神经性疼痛是一种使人衰弱的慢性疾病，会严重造成个人和社会负担，其中激活的神经炎症是一个主要原因。胸腺基质淋巴细胞生成素 (TSLP) 和白细胞介素 (IL)-33 对于慢性炎症很重要。乙酸芳樟酯 (LA) 是薰衣草油的主要成分，通过 TSLP 信号传导具有抗炎特性。该研究的目的是研究 LA 如何调节坐骨神经损伤 (SNI) 后的机械性痛觉过敏。成年 Sprague-Dawley 雄性大鼠分为 3 组：对照组、SNI 组和 SNI 加 LA 组。LA 在 SNI 前一天腹膜内给药。通过校准钳测试评估疼痛行为测试。同侧坐骨神经 (SN)，收集背根神经节 (DRG) 和脊髓用于免疫荧光染色和蛋白质印迹分析。SNI大鼠自手术后对机械刺激的痛觉过敏反应更加敏感，并伴有脊髓神经胶质细胞反应和DRG神经胶质相互作用。LA可以减轻痛觉、促炎细胞因子和降低TSLP/TSLPR复合物的表达。此外，LA 可以通过减少 IL-33 信号传导来减轻炎症。这项研究首次表明 LA 可以在神经损伤后通过 TSLP/TSLPR 和 IL-33 信号调节疼痛。LA可以减轻痛觉、促炎细胞因子和降低TSLP/TSLPR复合物的表达。此外，LA 可以通过减少 IL-33 信号传导来减轻炎症。这项研究首次表明 LA 可以在神经损伤后通过 TSLP/TSLPR 和 IL-33 信号调节疼痛。LA可以减轻痛觉、促炎细胞因子和降低TSLP/TSLPR复合物的表达。此外，LA 可以通过减少 IL-33 信号传导来减轻炎症。这项研究首次表明 LA 可以在神经损伤后通过 TSLP/TSLPR 和 IL-33 信号调节疼痛。