Yeast β-glucan (YG) possess an extensive range of biological activities, such as the inhibition of oxidation, but the poor water solubility of macromolecular YG limits its application. In this study, through the combined degradation of ultrasonic waves and H₂O₂, and the optimization of the main process parameters for solubilizing YG by response surface methodology (RSM), a new product of YG_UH was generated. The molecular weight, structural characteristics and degradation kinetics before and after solubilization were evaluated. The results showed that the optimal solubilization conditions were reaction time: 4 h, ultrasonic power: 3 W/mL, H₂O₂ concentration: 24 %. Under these conditions, ultrasound-assisted H₂O₂ increased the solubility (from 13.60 % to 70.00 %) and reduced molecular weight (from 6.73 × 10⁶ Da to 1.22 × 10⁶ Da). Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), Congo red (CR), scanning electron microscopy (SEM) revealed that ultrasound-assisted H₂O₂ increased the conformation's flexibility greatly, without changing the main structure of YG. More importantly, solubilization of YG improved free radical scavenging activity with YG_UH exhibiting the highest levels of DPPH and ABTS⁺ free radical scavenging activity. These results revealed that ultrasound-assisted H₂O₂ degradation could be a suitable way to increase the solubility of YG for producing value-added YG.

酵母β-葡聚糖（YG）具有广泛的生物活性，例如抑制氧化，但大分子YG的水溶性差限制了其应用。本研究通过超声波和H ₂ O ₂联合降解，并采用响应面法（RSM）优化增溶YG的主要工艺参数，生成了新产品YG _UH。评估了增溶前后的分子量、结构特征和降解动力学。结果表明，最佳增溶条件为反应时间：4 h，超声功率：3 W/mL，H ₂ O ₂浓度：24 %。在这些条件下，超声辅助 H₂ O ₂增加了溶解度（从 13.60 % 到 70.00 %）并降低了分子量（从 6.73 × 10 ⁶ Da 到 1.22 × 10 ⁶ Da）。傅里叶变换红外光谱(FTIR)、核磁共振(NMR)、刚果红(CR)、扫描电子显微镜(SEM)表明，超声辅助的H ₂ O ₂大大增加了构象的柔韧性，而没有改变YG的主要结构。更重要的是，YG 的增溶提高了自由基清除活性，YG _UH表现出最高水平的 DPPH 和 ABTS ⁺自由基清除活性。这些结果表明，超声辅助的 H ₂ O ₂降解可能是增加 YG 溶解度以生产增值 YG 的合适方法。