A reactive fluorescent “turn-on” probe (di-PIP) with imine-linked dual phenanthro[9,10-d]imidazole luminophore have been conveniently prepared as an Al³⁺ and H⁺ dual functional receptor. di-PIP displayed high selectivity and sensitivity towards Al³⁺ ion in DMF/HEPES accompanied by fluorescence blue-shift and a good linear relationship as well as a low detection limit of 30.5 nmol·L^–1, which can root from the synergetic functions of the decomposition reaction of di-PIP promoted by acidic Al³⁺ and the coordination effect between decomposition product and Al³⁺. Intriguingly, it was found that hydrogen ion H⁺ can be sufficient for simulating the fluorescence enhancing of di-PIP. ¹H NMR titration and MS analyses for elucidation of the intermediate structure further revealed that the acid-triggered decomposition reaction resulted in the rapid, and sensitive sensing to Al³⁺ and H⁺. In addition, the probe di-PIP could be successfully applied to the detection of Al³⁺ in real water samples, and also utilized to visualize Al³⁺ and H⁺ in the living cells.

具有亚胺连接的双菲[9,10- d ]咪唑发光团的反应性荧光“开启”探针 ( di-PIP )已被方便地制备为 Al ³⁺和 H ⁺双功能受体。di-PIP对DMF/HEPES中的Al ³⁺离子具有高选择性和灵敏度，并伴有荧光蓝移和良好的线性关系，检出限低至30.5 nmol·L ^–1，这可以归因于协同作用酸性Al ^3+促进di-PIP的分解反应及分解产物与Al ^{3+的配位作用}. 有趣的是，发现氢离子H ⁺足以模拟di-PIP的荧光增强。^{用于阐明中间结构的1} H NMR 滴定和 MS 分析进一步表明，酸引发的分解反应导致对 Al ³⁺和 H ⁺的快速、灵敏的检测。此外，探针di-PIP可成功应用于实际水样中Al ³⁺的检测，也可用于活细胞中Al ³⁺和H ^{+的可视化。}