Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic purposes. Affinity capillary electrophoresis (ACE) is one technique that can be used to examine the binding between drugs and serum proteins, or other agents found in serum or blood. This article will review the basic principles of ACE, along with related affinity-based capillary electrophoresis (CE) methods, and examine recent developments that have occurred in this field as related to the characterization of drug–protein interactions. An overview will be given of the various formats that can be used in ACE and CE for such work, including the relative advantages or weaknesses of each approach. Various applications of ACE and affinity-based CE methods for the analysis of drug interactions with serum proteins and other binding agents will also be presented. Applications of ACE and related techniques that will be discussed include drug interaction studies with serum agents, chiral drug separations employing serum proteins, and the use of CE in hybrid methods to characterize drug binding with serum proteins.

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通过亲和毛细管电泳分析药物与血清蛋白和相关结合剂的相互作用：综述

血清蛋白等生物分子可以与体内的药物相互作用，影响药物的药效。分析这些相互作用的具体而精确的方法对于药物开发或监测以及诊断目的至关重要。亲和毛细管电泳 (ACE) 是一种可用于检查药物与血清蛋白或血清或血液中发现的其他试剂之间结合的技术。本文将回顾 ACE 的基本原理，以及相关的基于亲和力的毛细管电泳 (CE) 方法，并研究该领域与药物-蛋白质相互作用表征相关的最新进展。将概述可在 ACE 和 CE 中用于此类工作的各种格式，包括每种方法的相对优势或劣势。还将介绍 ACE 和基于亲和力的 CE 方法在药物与血清蛋白和其他结合剂相互作用分析中的各种应用。将讨论的 ACE 和相关技术的应用包括与血清试剂的药物相互作用研究、使用血清蛋白的手性药物分离，以及 CE 在混合方法中的使用以表征药物与血清蛋白的结合。