Fracture healing is a complicated process containing the regulation of cellular process. It has been reported that circRNAs are involved in fracture healing. Our study aims to explore the role and mechanism of circ_C4orf36 in fracture healing. Here, we found that the expressions of Circ_C4orf36 and VEGFA were increased during osteoblast differentiation in MC3T3-E1 cells. Circ_C4orf36 overexpression could accelerate the proliferation and migration, as well as osteoblast differentiation in MC3T3-E1 cells, as well as increased ALP activity and osteogenic markers (Runx2, OCN) via upregulating VEGFA expression. Mechanistically, circ_C4orf36 facilitated the expression of VEGFA by recruiting EIF4A3. Taken together, our results elucidated that circ_C4orf6 promoted the migration, proliferation and osteoblast differentiation in BMSCs by upregulating VEGFA, which indicated that circ_C4orf36 might be a potential target in fracture healing treatment.

骨折愈合是一个复杂的过程，包含细胞过程的调节。据报道，circRNA参与骨折愈合。我们的研究旨在探讨circ_C4orf36在骨折愈合中的作用和机制。在此，我们发现 MC3T3-E1 细胞成骨细胞分化过程中Circ_C4orf36和VEGFA的表达增加。Circ_C4orf36过表达可以加速 MC3T3-E1 细胞的增殖和迁移以及成骨细胞分化，并通过上调 VEGFA 表达增加 ALP 活性和成骨标志物（Runx2、OCN ）。从机制上讲，circ_C4orf36促进了表达VEGFA通过招募 EIF4A3。综上所述，我们的结果阐明了circ_C4orf6通过上调VEGFA促进 BMSC 的迁移、增殖和成骨细胞分化，这表明circ_C4orf36可能是骨折愈合治疗的潜在靶点。