A novel thiadiazole functionalized schiff base chemoreceptor (E)-2,4-dichloro-6-(((5-mercapto-1,3,4-thiadiazol-2-yl)imino)methyl)phenol (SB-1) has been synthesized and characterized spectroscopically by using various techniques. Its photophysical behaviour was scanned towards a variety of metal ions in mixed aqueous media. The chemosensor (SB-1) displayed excellent selectivity towards Cu²⁺ ion through fluorescent diminishment (turn-off phenomenon). Colorimetric analyses showed a rapid colour change from yellow to dark red under visible light upon addition of Cu²⁺ ions. Interestingly, the original yellow colour reappeared back instantly after the addition of EDTA²⁻ anions, thus confirming the reversible nature of SB-1. Competitive experiments validated no interference from the other co-existing metal ions in the recognition process of SB-1 towards Cu²⁺ ion. Job’s plot confirmed 1:1 binding stoichiometry between SB-1 and Cu²⁺ ion with the binding constant value of 3.87 × 10⁴ M^− 1. The limit of detection was determined to be 1.01 × 10^− 7 M suggesting good sensitivity of SB-1 towards Cu²⁺ ions. Furthermore, pH-dependent UV-Vis spectral behaviour of SB-1 confirmed that it could act as an effective optical pH-sensor for highly acidic environment as well. Portable nature of probe SB-1 was explored by fabricating “easy-to-use” paper test strips, which allow robust and rapid detection of Cu²⁺ ions. Based on the multi-responsive properties of SB-1, a ‘NOR’ logic gate was constructed by applying Cu²⁺ and EDTA²⁻ as chemical inputs (ln1: Cu²⁺, ln2: EDTA²⁻) while emission intensity observed at 560 nm was considered as output signal (O1). DFT optimized geometries confirmed that chemosensor SB-1 exists in Azo form (Enol form) in its ground state. Molecular docking of the SB-1 and its copper complex, into the binding site of TRK protein tyrosine kinase (PDB: 1t46) was also carried out to explore their biological activity and their potential use as TRK inhibitors.

一种新型噻二唑官​​能化席夫碱化学受体 (E)-2,4-二氯-6-(((5-巯基-1,3,4-噻二唑-2-基)亚氨基)甲基)苯酚(SB-1 )通过使用各种技术进行光谱合成和表征。针对混合水介质中的各种金属离子扫描其光物理行为。化学传感器(SB-1)通过荧光减弱（关闭现象）显示出对 Cu ^{2+离子的出色选择性。比色分析显示在可见光下添加 Cu 2+}离子后颜色从黄色快速变为深红色。^{有趣的是，加入 EDTA 2}后，原来的黄色立即重新出现-阴离子，从而证实了SB-1的可逆性。竞争性实验证实在SB-1对Cu ²⁺离子的识别过程中没有其他共存金属离子的干扰。乔布斯图证实了SB-1和 Cu ²⁺离子之间的 1:1 结合化学计量，结合常数值为 3.87 × 10 ⁴  M ^{− 1}。检测限确定为 1.01 × 10 ^{− 7}  M，表明SB-1对 Cu ²⁺离子具有良好的灵敏度。此外， SB-1的 pH 依赖性紫外-可见光谱行为证实它也可以作为高酸性环境的有效光学 pH 传感器。通过制造“易于使用”的纸质试纸探索了探针SB-1的便携特性，该试纸允许对 Cu ²⁺离子进行稳健且快速的检测。基于SB-1的多响应特性，通过应用 Cu ²⁺和 EDTA ²⁻作为化学输入（ln1：Cu ²⁺，ln2：EDTA ²⁻ ）构建了“或非”逻辑门，同时观察到发射强度560 nm 被认为是输出信号 (O1)。DFT 优化的几何形状证实化学传感器SB-1在其基态以偶氮形式（烯醇形式）存在。SB-1的分子对接及其铜配合物进入 TRK 蛋白酪氨酸激酶 (PDB: 1t46) 的结合位点，以探索它们的生物活性及其作为 TRK 抑制剂的潜在用途。