Developing inexpensive nicotinamide cofactor biomimetics to replace the expensive NAD(P)/H cofactors is an ongoing research activity. Here we present mutational studies on a thermostable glucose dehydrogenase from Saccharolobus solfataricus (SsGDH) using a novel set of synthetic cofactors. Furthermore, we show the successful oxidation of a variety of different sugars in the context of cofactor regeneration. This combined approach resulted in an 160-fold improved system compared to the native enzyme with the standard biomimetic BNA⁺. These findings pave the way towards competitive industrial utilization of artificial cofactor regeneration systems.

中文翻译：

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促进人工烟酰胺辅因子系统

开发廉价的烟酰胺辅因子仿生剂来替代昂贵的 NAD(P)/H 辅因子是一项正在进行的研究活动。在这里，我们使用一组新的合成辅因子对来自Saccharolobus solfataricus ( Ss GDH)的热稳定葡萄糖脱氢酶进行突变研究。此外，我们展示了在辅因子再生的背景下成功氧化各种不同的糖。^{与具有标准仿生 BNA +}的天然酶相比，这种组合方法产生了 160 倍改进的系统。这些发现为人工辅因子再生系统的竞争性工业利用铺平了道路。