当前位置: X-MOL 学术Environ. Sci. Technol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Improving the Quantification of Cyanotoxins Using a Mass Balance-Based Effective Concentration-Equivalent Concentration Approach
Environmental Science & Technology ( IF 11.4 ) Pub Date : 2022-10-04 , DOI: 10.1021/acs.est.2c03194
Ai Jia 1 , Yingbo C Guo 1 , George D Di Giovanni 1 , Matthew D Prescott 1 , Wei L Li 1 , Eduardo A Garcia 1
Affiliation  

Two commonly used methods for cyanotoxin analysis are enzyme-linked immunosorbent assay (ELISA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). Each method has its advantages and disadvantages, and discrepancies are commonly observed between the two methods due to various factors including the ELISA antibody cross-reacting to different cyanotoxin congeners. However, reliable cyanotoxin monitoring methods and accurate interpretation of results are needed for water utilities to guide recreational water planning and drinking water treatment operations. In this study, we explored an innovative “effective concentration-equivalent concentration” (EC-EQ) approach to improve the interpretation of ELISA results and the comparison to LC-MS/MS results. The precision of ELISA results was first improved by reporting the sample ECs and EQs derived from their ELISA dose curves. Concentrations of each cyanotoxin as measured by LC-MS/MS were then combined with their respective ELISA cross-reactivities to calculate their theoretical ELISA responses. Finally, instead of comparing the results from the two methods directly, the equivalent concentration based on one single reference cyanotoxin was used for reporting and comparison. This integrated mass balance-based approach provides a more reliable interpretation of results by considering the reactivity differences between toxins as well as their mixture effects. This approach has been successfully applied to microcystin (one main group of cyanotoxins) standard mixtures and cyanobacterial bloom samples to interpret and compare their ELISA and LC-MS/MS detection results. The study provides guidance to utilities on how to obtain more accurate cyanotoxin monitoring results and better understand the discrepancy between the two methods.

中文翻译:

使用基于质量平衡的有效浓度等效浓度方法改进蓝藻毒素的定量

两种常用的蓝藻毒素分析方法是酶联免疫吸附试验 (ELISA) 和液相色谱-串联质谱 (LC-MS/MS)。每种方法都有其优点和缺点,由于各种因素,包括 ELISA 抗体与不同的蓝藻毒素同源物发生交叉反应,两种方法之间通常存在差异。然而,供水公司需要可靠的蓝藻毒素监测方法和准确的结果解释来指导娱乐用水规划和饮用水处理操作。在这项研究中,我们探索了一种创新的“有效浓度等效浓度”(EC-EQ)方法来改进 ELISA 结果的解释以及与 LC-MS/MS 结果的比较。首先通过报告从 ELISA 剂量曲线得出的样本 EC 和 EQ 来提高 ELISA 结果的精度。然后将通过 LC-MS/MS 测量的每种蓝藻毒素的浓度与它们各自的 ELISA 交叉反应性相结合,以计算它们的理论 ELISA 反应。最后,不是直接比较两种方法的结果,而是使用基于单一参考蓝藻毒素的当量浓度进行报告和比较。这种基于质量平衡的综合方法通过考虑毒素之间的反应性差异及其混合物效应,为结果提供了更可靠的解释。这种方法已成功应用于微囊藻毒素(一种主要的蓝藻毒素)标准混合物和蓝藻水华样品,以解释和比较它们的 ELISA 和 LC-MS/MS 检测结果。该研究为公用事业公司如何获得更准确的蓝藻毒素监测结果和更好地了解两种方法之间的差异提供了指导。
更新日期:2022-10-04
down
wechat
bug