Using filamentous fungi to capture unicellular microalgae is an effective way for microalgae recovery in water treatment. Here, fungi Aspergillus flavus ZJ-1 and microalgae Chlorella vulgaris WZ-1 isolated from a copper tailings pond were used to study the capture effect of ZJ-1 on WZ-1. The highest capture efficiency (97.85%) was obtained within 6 h under the optimized conditions of 30 °C, 150 rpm, fungi-algae biomass ratio of 2.24:1, and initial pH of 9.24 in microalgae medium. The formed fungi-algae pellets (FAPs) were further used to remove Cu(II) from aqueous solution. Results showed that the FAPs formed at different capture times all adsorbed Cu(II) well, and the PAFs formed within 2 h (PAFs_2 h) exhibited the highest Cu(II) adsorption capacity (80.42 mg·g⁻¹). SEM images showed that Cu(II) caused a change in the internal structure of PAFs_2 h from loose to compact, the mycelium shrunk, and the microalgal cells were concave. Cu(II) adsorption by PAFs_2 h was well conformed to the pseudo-second-order kinetics and the Langmuir isotherm (123.61 mg·g⁻¹ of theoretically maximum adsorption capacity). This work opens a way for applying FAPs in the remediation of heavy metal-contaminated wastewater, and the metal adsorption effect was determined by the capture amount of microalgae.

利用丝状真菌捕获单细胞微藻是水处理中微藻回收的有效途径。在这里，从铜尾矿池中分离出的真菌黄曲霉ZJ-1 和微藻小球藻WZ-1 用于研究 ZJ-1 对 WZ-1 的捕获效果。在 30 ℃、150 rpm、菌藻生物量比为 2.24:1 和初始 pH 为 9.24 的微藻培养基中的优化条件下，在 6 h 内获得了最高的捕获效率（97.85%）。形成的真菌-藻类颗粒 (FAP) 进一步用于从水溶液中去除 Cu(II)。结果表明，不同捕获时间形成的 FAPs 均能很好地吸附 Cu(II)，且 PAFs 在 2 h 内形成（PAFs _{2 h}) 表现出最高的Cu(II) 吸附容量（80.42 mg·g ^-1）。SEM图像显示Cu(II)导致PAFs内部结构在_{2 h}后由松散变为致密，菌丝体收缩，微藻细胞呈凹形。_{PAFs 2 h}对Cu(II) 的吸附非常符合准二级动力学和Langmuir 等温线（理论上最大吸附容量为123.61 mg·g ^-1）。该工作为应用FAPs修复重金属污染废水开辟了一条途径，金属吸附效果由微藻的捕获量决定。