Cadmium (Cd) isotope fractionation patterns within soil profiles and the underlying mechanisms remain unclear and poorly documented. Here, Cd concentrations and isotope compositions of metal ore, surface soils and soil profile samples around a lead-zinc mine in southwest China were determined, and the relationships between soil properties and Cd isotope fractionation within the soil profiles were investigated. Cadmium concentrations of eleven surface soil samples were 0.49–66.1 mg kg⁻¹ and the samples with high Cd concentrations had Cd isotope compositions similar to the metal ore (δ^114/110Cd = 0.02‰), indicating that mining activity was the main Cd source at the study areas. Within three soil profiles with different Cd pollution levels the δ^114/110Cd values gradually increased with increasing depth from 0 to 40 cm (Δ^114/110Cd = 0.08–0.18‰), reaching a maximum at 30–40 cm depth, and then remained fairly constant or decreased with increasing soil depth below 40 cm. Soil δ^114/110Cd values were negatively correlated with free iron and manganese oxides contents, which decreased at 0–40 cm depth then increased below 40 cm. This indicates that light Cd isotopes within 0–40 cm depth preferentially migrated downward with free iron and manganese oxides, leaving the soils at a depth of 0–40 cm enriched in heavy Cd isotopes. At 40–90 cm depth the preferential retention of heavy Cd isotopes by hydroxides may be responsible for the gradual decrease in δ^114/110Cd values with increasing soil depth. These observations demonstrate that the vertical migration of Cd can induce detectable isotope fractionation within soil profiles and alter the δ^114/110Cd values including those of the surface soils. Our study highlights the need to consider Cd mobilization and transport in soil profiles when tracing metal sources using isotope techniques.