Paraquat (PQ) is one of the most commonly used herbicides, but it has polluted the environment and threatened human health through extensive and improper usage. Here, a new naked-eye PQ immunochromatographic strip was developed to recognize PQ in domestic water and real human samples within 10 min based on a novel custom-designed anti-PQ antibody. The PQ test strip could recognize PQ at a concentration as low as 10 ng/ml, reaching the high-efficiency time-of-flight mass spectrometry detection level and identifying trace amounts of PQ in samples treated with a diquat (DQ) and PQ mixture. Notably, both the performance evaluation and clinical trial of the proposed PQ strips were validated in multiple hospitals and public health agencies. Taken together, our study firstly provide the clinical PQ-targeted colloidal gold immunochromatographic test strip designed both for environment water and human sample detection with multiple advantages, which are ready for environmental monitoring and clinical practice.

百草枯 (PQ) 是最常用的除草剂之一，但由于广泛和不当使用，已污染环境，威胁人类健康。在这里，基于新型定制设计的抗 PQ 抗体，开发了一种新的裸眼 PQ 免疫层析条带，可在 10 分钟内识别生活用水和真实人体样品中的 PQ。PQ试纸可识别浓度低至10 ng/ml的PQ，达到高效飞行时间质谱检测水平，可识别敌草快（DQ）和PQ混合物处理的样品中的痕量PQ . 值得注意的是，所提议的 PQ 试纸的性能评估和临床试验都在多家医院和公共卫生机构中得到了验证。综合起来，