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Detection of lymphocystis disease virus (LCDV) from yellowbar angelfish (Pomacanthus maculosus Forsskål, 1775) in Iran: Histopathological and phylogenetic analysis
Aquaculture ( IF 4.5 ) Pub Date : 2022-09-24 , DOI: 10.1016/j.aquaculture.2022.738862
Hooman Rahmati-Holasoo, Arash Ghalyanchilangeroudi, Zahra Ziafati Kafi, Amin Marandi, Sara Shokrpoor, Bahram Imantalab, Hosseinali Ebrahimzadeh Mousavi

Lymphocystis disease (LCD) is a benign viral disease characterized by the appearance of hypertrophic fibroblastic cells in the connective tissues of fish. In December 2021, a group of yellowbar angelfish (Pomacanthus maculosus Forsskål, 1775) which had significant mortalities (10%) and showed whitish nodules on the fins and skin, were packed in water-filled plastic bags, supplied with oxygen, and transported to the Ornamental Fish Clinic, Faculty of Veterinary Medicine, University of Tehran (Tehran, Iran). Following the clinical inspection and observation of whitish clusters of nodules mostly on the fins and skin by stereomicroscopy, wet smears of skin and fins revealed numerous hypertrophic lymphocystis cells. Histopathological study of the whitish nodules in skin and fins revealed many hypertrophic cells with thick hyaline capsules, irregular nucleus, and multiple intracytoplasmic inclusion bodies. In addition to the extension of hypertrophic lymphocystis cells' growth until the superficial muscle layer, infiltration of inflammatory cells and hemorrhage between hypertrophic cells are also observed. The hyaline capsule of hypertrophic fibroblasts stained with the PAS. Molecular characterization (PCR) confirmed the presence of Lymphocystis disease virus (LCDV) in the external nodules and the internal organs homogenate of the infected fish. Sequence analysis of the MCP gene of the detected virus (IR_LCDV_RGZIS) assigned it to genogroup 5. This is the first detection and characterization of LCDV in yellowbar angelfish (Pomacanthus maculosus) in Iran.



中文翻译:

伊朗黄鳍神仙鱼 (Pomacanthus maculosus Forsskål, 1775) 淋巴囊肿病病毒 (LCDV) 的检测:组织病理学和系统发育分析

淋巴囊肿病 (LCD) 是一种良性病毒性疾病,其特征是鱼的结缔组织中出现肥大的成纤维细胞。2021年12月,一群黄鳍神仙鱼(Pomacanthus maculosus )Forsskål, 1775) 死亡率很高 (10%) 并在鳍和皮肤上显示白色结节,被装在装满水的塑料袋中,提供氧气,然后运送到大学兽医学院观赏鱼诊所德黑兰(伊朗德黑兰)。体视显微镜下临床检查和观察主要在鳍和皮肤上的白色结节簇,皮肤和鳍的湿涂片显示许多肥大的淋巴囊肿细胞。皮肤和鳍的白色结节的组织病理学研究显示许多肥大的细胞具有厚的透明被膜、不规则的细胞核和多个胞浆内包涵体。除了将肥大淋巴囊细胞的生长延伸到浅层肌肉层外,还观察到炎症细胞的浸润和肥大细胞之间的出血。用 PAS 染色的肥大成纤维细胞的透明囊。分子特征 (PCR) 证实了感染鱼的外部结节和内部器官匀浆中存在淋巴囊虫病病毒 (LCDV)。序列分析检测到的病毒的MCP基因 (IR_LCDV_RGZIS) 将其分配到基因组 5。这是伊朗首次在黄鳍神仙鱼 ( Pomacanthus maculosus ) 中检测和表征 LCDV。

更新日期:2022-09-29
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