Pancreatic cancer is the 7th leading cause of cancer death worldwide, and its incidence and mortality rate have been on the rise in recent years in Western developed countries. The specificity of the disease and the lack of appropriate treatments have resulted in a 5-year overall survival rate of only 9%. In this study, we conducted a study based on the TCGA database and GEO database and analyzed using the energy metabolism gene set to establish a prognostic model with the least absolute shrinkage and selection operator to identify 7-genes prognostic signature, and the gene expression was verified by Real-time PCR. The model was validated using a risk score calculation, and the OS rates of the 7 genes were analyzed using one-way Cox regression. The prognostic relationship between vesicle-associated membrane protein 2 (VAMP2) and pancreatic cancer patients was analyzed by OS and progression-free survival, and the prognosis was found to be significantly worse in the high-expression group. A Nomogram showed that VAMP2 was an independent prognostic factor in pancreatic cancer. Gene set enrichment analysis showed that VAMP2 upregulation was enriched in pathways associated with immune response and that VAMP2 downregulation was enriched in metabolism-related pathways. The association of VAMP2 with immune cell infiltration was analyzed for the enrichment results, and VAMP2 was found to be positively associated with all 6 immune cells. The results of this study suggest that VAMP2 is an independent prognostic factor associated with energy metabolism in pancreatic cancer and may be involved in the immune response.

胰腺癌是全球第七大癌症死亡原因，近年来在西方发达国家其发病率和死亡率均呈上升趋势。该疾病的特异性和缺乏适当的治疗导致 5 年总生存率仅为 9%。本研究基于 TCGA 数据库和 GEO 数据库进行研究，利用能量代谢基因集进行分析，建立具有最小绝对收缩和选择算子的预后模型，识别 7 基因预后特征，基因表达为通过实时 PCR 验证。使用风险评分计算验证模型，并使用单向 Cox 回归分析 7 个基因的 OS 率。通过OS和无进展生存期分析囊泡相关膜蛋白2（VAMP2）与胰腺癌患者的预后关系，发现高表达组的预后明显更差。列线图显示 VAMP2 是胰腺癌的独立预后因素。基因集富集分析表明，VAMP2 上调富集于与免疫反应相关的通路，而 VAMP2 下调富集于代谢相关通路。富集结果分析了 VAMP2 与免疫细胞浸润的关联，发现 VAMP2 与所有 6 种免疫细胞呈正相关。