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Mechanism of Dexmedetomidine Intervention on Neurogenic Inflammation in Cognitive Impairment Rats after Partial Hepatectomy
Computational and Mathematical Methods in Medicine ( IF 2.809 ) Pub Date : 2022-9-29 , DOI: 10.1155/2022/8714221 Zitan Zhang 1 , Huiqun Jia 1
Computational and Mathematical Methods in Medicine ( IF 2.809 ) Pub Date : 2022-9-29 , DOI: 10.1155/2022/8714221 Zitan Zhang 1 , Huiqun Jia 1
Affiliation
Objective. To study the effect of dexmedetomidine on cognitive function in rats with cognitive impairment after partial hepatectomy and its mechanism. Methods. 60 SD rats were randomly divided into 4 groups (): blank control group (CG group), sham operation group (Sham group), cognitive impairment model group (POCD group), and dexmedetomidine + cognitive impairment model group (DEX group). Rats in the POCD group underwent left lobe hepatectomy and intraperitoneal injection of the same amount of normal saline after resuscitation. Rats in the DEX group underwent left lobe hepatectomy and intraperitoneal injection of dexmedetomidine 50 μg/kg. Group CG was not operated on and the same amount of normal saline was injected intraperitoneally. In the Sham group, liver resection was not allowed after the abdominal incision, and normal saline was injected intraperitoneally. Rats were injected every 24 hours for 5 consecutive days. Morris water maze (MWM) were used to evaluate the effects of dexmedetomidine on learning and memory ability of POCD rats. TUNEL method was used to detect apoptotic neurons in the hippocampus. INOS, Arg-1, IL-6, and TNF-αexpression levels were detected. Western blot detects the expression level of TNF-α, Bcl-2, and NF-κB protein. Result. Compared with the CG group, the escape latency of the other three groups was prolonged on the 5th day after the operation, and the number of crossing the platform was reduced. Compared with the Sham group, the escape latency of the POCD group and DEX group was significantly prolonged, and the number of crossing the platform was significantly reduced on day 5 (). Compared with the POCD group, the DEX group shortened the escape latency and increased the number of crossing the platform on the 5th day (). It shows that the spatial learning and memory function of rats has been restored to a certain extent.The number of iNOS and Arg-1 positive cells in the POCD group and DEX group was higher than that in the control group, and the number of Arg-1 positive cells in the DEX group was higher than that in the POCD group (). Western blot results the expression of Bcl-2 and NF-κB protein in POCD group, and DEX group was higher than that of the sham group (). The expression of Bcl-2 and NF-κB protein was the most in POCD group. The expression of Bcl-2 and NF-κB protein in DEX group was lower than that in POCD group (). Conclusion. Behavioral results showed that the learning and cognitive ability of POCD model rats after hepatectomy was impaired, and inflammatory factors and activated microglia were found in the hippocampus of POCD rats. Dexmedetomidine may improve the brain function of POCD rats by inhibiting neuronal apoptosis,partly through NF-κB apoptosis pathway.
中文翻译:
右美托咪定干预认知障碍大鼠肝部分切除术后神经源性炎症的机制
客观。研究右美托咪定对肝部分切除术后认知障碍大鼠认知功能的影响及其机制。方法。60只SD大鼠随机分为4组():空白对照组(CG组)、假手术组(Sham组)、认知障碍模型组(POCD组)、右美托咪定+认知障碍模型组(DEX组)。POCD组大鼠行左叶肝切除,复苏后腹腔注射等量生理盐水。DEX组大鼠行左叶肝切除并腹腔注射右美托咪定 50μ克/公斤。CG组不做手术,腹腔注射等量生理盐水。Sham组腹腔切开后不进行肝切除,腹腔注射生理盐水。大鼠每 24 小时注射一次,连续 5 天。采用Morris水迷宫(MWM)评价右美托咪定对POCD大鼠学习记忆能力的影响。TUNEL法用于检测海马中凋亡的神经元。检测到INOS、Arg-1、IL-6 和 TNF- α表达水平。Western blot 检测 TNF - α、Bcl-2 和 NF- κ B 蛋白的表达水平。结果. 与CG组相比,术后第5天其他三组的逃逸潜伏期延长,过台次数减少。与Sham组相比,POCD组和DEX组的逃逸潜伏期明显延长,第5天越台次数明显减少()。与 POCD 组相比,DEX 组缩短了逃逸潜伏期,增加了第 5 天过平台的次数()。说明大鼠空间学习记忆功能得到了一定程度的恢复。POCD组和DEX组iNOS和Arg-1阳性细胞数均高于对照组,Arg DEX组-1阳性细胞高于POCD组()。Western blot结果显示POCD组Bcl-2和NF-κB蛋白表达,DEX组高于假手术组()。POCD组Bcl-2和NF-κB蛋白表达最多。DEX组Bcl-2和NF-κB蛋白表达低于POCD组()。 结论。行为学结果显示,POCD模型大鼠肝切除术后学习和认知能力受损,POCD大鼠海马内发现炎症因子和活化小胶质细胞。右美托咪定可能通过抑制神经元凋亡改善POCD大鼠脑功能,部分通过NF-κB凋亡通路。
更新日期:2022-09-29
中文翻译:
右美托咪定干预认知障碍大鼠肝部分切除术后神经源性炎症的机制
客观。研究右美托咪定对肝部分切除术后认知障碍大鼠认知功能的影响及其机制。方法。60只SD大鼠随机分为4组():空白对照组(CG组)、假手术组(Sham组)、认知障碍模型组(POCD组)、右美托咪定+认知障碍模型组(DEX组)。POCD组大鼠行左叶肝切除,复苏后腹腔注射等量生理盐水。DEX组大鼠行左叶肝切除并腹腔注射右美托咪定 50μ克/公斤。CG组不做手术,腹腔注射等量生理盐水。Sham组腹腔切开后不进行肝切除,腹腔注射生理盐水。大鼠每 24 小时注射一次,连续 5 天。采用Morris水迷宫(MWM)评价右美托咪定对POCD大鼠学习记忆能力的影响。TUNEL法用于检测海马中凋亡的神经元。检测到INOS、Arg-1、IL-6 和 TNF- α表达水平。Western blot 检测 TNF - α、Bcl-2 和 NF- κ B 蛋白的表达水平。结果. 与CG组相比,术后第5天其他三组的逃逸潜伏期延长,过台次数减少。与Sham组相比,POCD组和DEX组的逃逸潜伏期明显延长,第5天越台次数明显减少()。与 POCD 组相比,DEX 组缩短了逃逸潜伏期,增加了第 5 天过平台的次数()。说明大鼠空间学习记忆功能得到了一定程度的恢复。POCD组和DEX组iNOS和Arg-1阳性细胞数均高于对照组,Arg DEX组-1阳性细胞高于POCD组()。Western blot结果显示POCD组Bcl-2和NF-κB蛋白表达,DEX组高于假手术组()。POCD组Bcl-2和NF-κB蛋白表达最多。DEX组Bcl-2和NF-κB蛋白表达低于POCD组()。 结论。行为学结果显示,POCD模型大鼠肝切除术后学习和认知能力受损,POCD大鼠海马内发现炎症因子和活化小胶质细胞。右美托咪定可能通过抑制神经元凋亡改善POCD大鼠脑功能,部分通过NF-κB凋亡通路。