Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.

中文翻译：

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一种具有竞争力的精确 CRISPR 方法，用于识别转录因子结合位点的适应度效应

在这里，我们描述了一种竞争性基因组编辑方法，该方法基于精确的 CRISPR 编辑，使用具有原始或改变序列的模板库和序列标签，可以直接比较原始细胞和突变细胞。以 MYC 致癌基因为例，我们确定了重要的转录靶点，并表明 MYC 靶基因启动子的 E-box 突变会降低细胞适应性。