Exploration of the mechanisms of cadmium (Cd) activation mediated by the rhizosphere process is important to advance our understanding of Cd accumulation in plants. In this study, two oilseed rape cultivars (L338, L351) with varied Cd accumulation traits were applied and the responses of their rhizosphere ecology to Cd stress were investigated by metabolome and microbiome. The results showed that shoot Cd accumulations in L338 accounted for 54.16% and 64.76% of those in L351 under low and high Cd contamination, respectively. Moreover, the cultivars response of rhizosphere process reflected that the lower pH and higher Cd mobility were assigned to the characters of L351, which were induced by the secretion of carboxylic acid (e.g. Acetaminophen cysteine, N-Fructosyl alliin) and the enrichment of bacterial taxa with the capacities of Cd resistant and activation (e.g. Sphingomonas, Flavobacterium, Neorhizobium, Altererythrobacter). Conclusively, the varied Cd accumulation traits of two oilseed rape cultivars were not only derived from the Cd transfer ability, it would be ascribed to Cd mobility regulated by rhizosphere processes as well. The results provide baseline data and a new perspective on the cultivar response of Cd accumulation, thus maintaining cleaner production of oilseed rape.

探索根际过程介导的镉 (Cd) 活化机制对于促进我们对植物中镉积累的理解具有重要意义。本研究以两种不同Cd积累性状的油菜品种（L338、L351）为研究对象，通过代谢组学和微生物组研究其根际生态对Cd胁迫的响应。结果表明，低镉污染和高镉污染下，L338茎秆Cd积累量分别占L351茎秆Cd积累量的54.16%和64.76%。此外，品种对根际过程的反应反映了较低的 pH 值和较高的 Cd 迁移率与 L351 的特征有关，这些特征是由羧酸（例如对乙酰氨基酚半胱氨酸、鞘氨醇单胞菌属、黄杆菌属、新根瘤菌属、交替红杆菌属）。总之，两种油菜品种的不同 Cd 积累性状不仅源于 Cd 转移能力，还归因于根际过程调节的 Cd 迁移率。该结果为 Cd 积累的品种响应提供了基线数据和新的视角，从而保持油菜的清洁生产。