With rapid growing of environmental contact infection, more and more attentions are focused on the precise and absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus on cold chain foods via point-of-care test (POCT). In this work, we propose a hydrogel-mediated reverse transcription loop-mediated isothermal amplification (RT-LAMP) for ultrafast and absolute quantification of SARS-CoV-2. Cross-linked hydrogel offers opportunities for digital single molecule amplification in nanoconfined spaces, facilitating the virus lysis, RNA reverse transcription and amplification process, which is about 3.4-fold faster than conventional bulk RT-LAMP. Ultrafast quantification of SARS-CoV-2 is accomplished in 15 min without virus pre-lysis and RNA extraction. The sensitivity can accurately quantify SARS-CoV-2 down to 0.5 copy/μL. Furthermore, the integrated system has an excellent specificity, reproducibility and storage stability, which can be also used to test SARS-CoV-2 on various cold chain fruits. The developed ultrafast and simple hydrogel RT-LAMP will be an enormous potential for surveillance of virus or other hazardous microbes in environmental, agricultural and food industry.

随着环境接触感染的快速增长，越来越多的注意力集中在通过即时检验（POCT）对冷链食品中严重急性呼吸系统综合症冠状病毒2（SARS-CoV-2）病毒进行精确和绝对定量。在这项工作中，我们提出了一种水凝胶介导的逆转录环介导等温扩增 (RT-LAMP)，用于 SARS-CoV-2 的超快和绝对定量。交联水凝胶提供了在纳米空间中进行数字单分子扩增的机会，促进了病毒裂解、RNA 逆转录和扩增过程，这比传统的散装 RT-LAMP 快约 3.4 倍。SARS-CoV-2 的超快速定量可在 15 分钟内完成，无需病毒预裂解和 RNA 提取。灵敏度可以准确量化 SARS-CoV-2 低至 0。5 拷贝/μL。此外，该集成系统具有优异的特异性、重现性和储存稳定性，也可用于对各种冷链水果进行SARS-CoV-2检测。开发的超快和简单的水凝胶 RT-LAMP 将在监测环境、农业和食品工业中的病毒或其他有害微生物方面具有巨大的潜力。