Green synthesis is an eco-friendly approach of nanoparticles fabrication gaining momentum among researches. Nanoparticles are used immensely, due to its small size, physical properties, orientation, which can apparently change the performance of any other material when in proximity. Antibacterial, antioxidant, antifungal, antiviral, anti-inflammatory activities of several nanoparticles capable of wound healing make it a appropriate module for wound dressing materials. Silver nanoparticles (SNPs) are recognized as well established antimicrobial and antiseptic agents, thus considering it as a promising candidate for wound healing process and other applications. Here we report an effective and biological approach of a novel thin film preparation based on polyvinyl alcohol (PVA) with SNPs generated within matrix using leaf extract of Aloe vera as a bioreducing agent. Successful incorporation of SNPs into the polymer matrix, which was confirmed by NTA analysis, TEM, SEM. The characterization results revealed that SNPs were found in range of 10–40 nm. Evaluation of antimicrobial activity on Escherichia coli (ATCC 39403), Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (clinical isolates), and Candida albicans (diploid fungus) using agar cup and disc diffusion method confirmed effective performance of the PVA-SNPs film. Average ZOI was observed as 3+2mm, 3+2mm, 4+1mm and 4+1mm respectively. Thin film produced is highly biocompatible to HaCat and L929 cell lines for a defined amount and hence can be used as wound dressing materials. The method used in this study is greener, simple, rapid, and cost effective for producing a biocompatible film with profoundly variable applications in food packaging and health care industries.

中文翻译：

---

聚乙烯醇薄膜基质中生物相容性生物银纳米颗粒的原位生长

绿色合成是一种环保的纳米颗粒制造方法，在研究中获得了蓬勃发展。纳米粒子因其尺寸小、物理特性和方向性而被广泛使用，当接近时，纳米粒子可以明显改变任何其他材料的性能。几种能够促进伤口愈合的纳米粒子的抗菌、抗氧化、抗真菌、抗病毒、抗炎活性使其成为伤口敷料材料的合适模块。银纳米颗粒（SNP）被认为是成熟的抗菌和防腐剂，因此被认为是伤口愈合过程和其他应用的有希望的候选者。在此，我们报告了一种基于聚乙烯醇 (PVA) 的新型薄膜制剂的有效生物学方法，该薄膜制剂使用植物叶提取物在基质内生成 SNP芦荟作为生物还原剂。SNP 成功掺入聚合物基质中，并通过 NTA 分析、TEM、SEM 证实。表征结果显示，SNP 的范围为 10-40 nm。抗菌活性评价大肠杆菌（ATCC 39403），金黄色葡萄球菌（ATCC 25923），肺炎克雷伯菌（临床分离株），以及使用琼脂杯和圆盘扩散法对白色念珠菌（二倍体真菌）证实了 PVA-SNPs 膜的有效性能。观察到平均ZOI分别为3+2mm、3+2mm、4+1mm和4+1mm。生产的薄膜与一定量的 HaCat 和 L929 细胞系具有高度生物相容性，因此可用作伤口敷料材料。本研究中使用的方法更环保、简单、快速且经济高效，可生产生物相容性薄膜，在食品包装和医疗保健行业具有广泛的应用。