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Nitrated Fatty-Acids Distribution in Storage Biomolecules during Arabidopsis thaliana Development
Antioxidants ( IF 7 ) Pub Date : 2022-09-21 , DOI: 10.3390/antiox11101869
Lorena Aranda-Caño 1 , Raquel Valderrama 1 , Mounira Chaki 1 , Juan C Begara-Morales 1 , Manuel Melguizo 2 , Juan B Barroso 1
Affiliation  

The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and derived species results in the formation of nitrated fatty acids (NO2-FAs). These signaling molecules can release NO, reversibly esterify with complex lipids, and modulate protein function through the post-translational modification called nitroalkylation. To date, NO2-FAs act as signaling molecules during plant development in plant systems and are involved in defense responses against abiotic stress conditions. In this work, the previously unknown storage biomolecules of NO2-FAs in Arabidopsis thaliana were identified. In addition, the distribution of NO2-FAs in storage biomolecules during plant development was determined, with phytosterol esters (SE) and TAGs being reservoir biomolecules in seeds, which were replaced by phospholipids and proteins in the vegetative, generative, and senescence stages. The detected esterified NO2-FAs were nitro-linolenic acid (NO2-Ln), nitro-oleic acid (NO2-OA), and nitro-linoleic acid (NO2-LA). The last two were detected for the first time in Arabidopsis. The levels of the three NO2-FAs that were esterified in both lipid and protein storage biomolecules showed a decreasing pattern throughout Arabidopsis development. Esterification of NO2-FAs in phospholipids and proteins highlights their involvement in both biomembrane dynamics and signaling processes, respectively, during Arabidopsis plant development.

中文翻译:

拟南芥发育过程中硝化脂肪酸在储存生物分子中的分布

多不饱和脂肪酸与一氧化氮 (NO) 和衍生物质的非酶促相互作用导致硝化脂肪酸 (NO 2 -FAs) 的形成。这些信号分子可以释放 NO,与复杂的脂质发生可逆的酯化反应,并通过称为硝基烷基化的翻译后修饰调节蛋白质功能。迄今为止,NO 2 -FAs 在植物系统的植物发育过程中充当信号分子,并参与针对非生物胁迫条件的防御反应。在这项工作中,确定了拟南芥中以前未知的 NO 2 -FAs储存生物分子。此外,NO 2的分布-测定了植物发育过程中储存生物分子中的 FAs,植物甾醇酯 (SE) 和 TAGs 是种子中的储存生物分子,在营养、生殖和衰老阶段被磷脂和蛋白质取代。检测到的酯化NO 2 -FAs为硝基亚麻酸(NO 2 -Ln)、硝基油酸(NO 2 -OA)和硝基亚油酸(NO 2 -LA)。后两种是首次在拟南芥中检测到。在脂质和蛋白质储存生物分子中酯化的三种 NO 2 -FAs 的水平在拟南芥的整个发育过程中呈下降趋势。NO 2的酯化-磷脂和蛋白质中的 FA 突出了它们在拟南芥植物发育过程中分别参与生物膜动力学和信号传导过程。
更新日期:2022-09-21
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