A green, simple and cost-effective, validated high-performance liquid chromatographic (HPLC) with UV detector has been developed an analytical method for the simultaneous determination of the most significant phenolic compounds viz. Gallic acid (GA), Quercetin (QC) and Ferulic acid (FA). The detection limit of GA, QC, FA was 0.303, 0.168, 0.0976 μg/mL and quantitation limit was 0.918, 0.051, 0.295 μg/mL, respectively. All parameters of planned validated method were carried out as per the ICH guidelines. This analytical method was applied to determine the phenolic compounds in fruits cultivated in India. Phenolic compounds in fruits extract were quantified using regression equations. This method can be scaled up to determine the alkaloids from several medicinal and poisonous plants samples in pharmaceutical, forensics and industrial areas.

中文翻译：

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使用 Brij-58 表面活性剂测定水果中酚类化合物的萃取-HPLC 绿色方法。

已开发出一种绿色、简单且具有成本效益且经过验证的带 UV 检测器的高效液相色谱 (HPLC) 分析方法，用于同时测定最重要的酚类化合物，即。没食子酸 (GA)、槲皮素 (QC) 和阿魏酸 (FA)。GA、QC、FA的检出限分别为0.303、0.168、0.0976 μg/mL，定量限分别为0.918、0.051、0.295 μg/mL。计划验证方法的所有参数均按照 ICH 指南执行。该分析方法用于测定印度种植的水果中的酚类化合物。使用回归方程对水果提取物中的酚类化合物进行量化。该方法可以扩大规模，以测定制药、法医学和工业领域中多种药用和有毒植物样品中的生物碱。