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Differential elasticity in lineage segregation of embryonic stem cells
arXiv - PHYS - Soft Condensed Matter Pub Date : 2022-09-20 , DOI: arxiv-2209.09707 Christine M. Ritter, Natascha Leijnse, Younes Farhangi Barooji, Joshua M. Brickman, Amin Doostmohammadi, Lene B. Oddershede
arXiv - PHYS - Soft Condensed Matter Pub Date : 2022-09-20 , DOI: arxiv-2209.09707 Christine M. Ritter, Natascha Leijnse, Younes Farhangi Barooji, Joshua M. Brickman, Amin Doostmohammadi, Lene B. Oddershede
The question of what guides lineage segregation is central to development,
where cellular differentiation leads to segregated cell populations destined
for specialized functions. Here, using optical tweezers measurements of mouse
embryonic stem cells (mESCs), we reveal a mechanical mechanism based on
differential elasticity in the second lineage segregation of the embryonic
inner cell mass into epiblast (EPI) cells - that will develop into the fetus -
and primitive endoderm (PrE) - which will form extraembryonic structures such
as the yolk sac. Remarkably, we find that these mechanical differences already
occur during priming and not just after a cell has committed to
differentiation. Specifically, we show that the mESCs are highly elastic
compared to any other reported cell type and that the PrE cells are
significantly more elastic than EPI-primed cells. Using a model of two cell
types differing only in elasticity we show that differential elasticity alone
can lead to segregation between cell types, suggesting that the mechanical
attributes of the cells contribute to the segregation process. Our findings
present differential elasticity as a previously unknown mechanical contributor
to the lineage segregation during the embryo morphogenesis.
中文翻译:
胚胎干细胞谱系分离的差异弹性
什么指导谱系分离的问题是发展的核心,其中细胞分化导致分离的细胞群注定具有特殊功能。在这里,使用光学镊子测量小鼠胚胎干细胞 (mESCs),我们揭示了一种机械机制,该机制基于胚胎内细胞团第二谱系分离成外胚层 (EPI) 细胞中的差异弹性 - 这将发育成胎儿 - 和原始内胚层 (PrE) - 将形成胚胎外结构,例如卵黄囊。值得注意的是,我们发现这些机械差异已经发生在启动过程中,而不仅仅是在细胞致力于分化之后。具体来说,我们表明,与任何其他报告的细胞类型相比,mESC 具有高弹性,并且 PrE 细胞比 EPI 引发的细胞更具弹性。使用仅弹性不同的两种细胞类型的模型,我们表明单独的差异弹性可以导致细胞类型之间的分离,这表明细胞的机械属性有助于分离过程。我们的研究结果表明差异弹性是胚胎形态发生过程中谱系分离的先前未知的机械贡献者。
更新日期:2022-09-21
中文翻译:
胚胎干细胞谱系分离的差异弹性
什么指导谱系分离的问题是发展的核心,其中细胞分化导致分离的细胞群注定具有特殊功能。在这里,使用光学镊子测量小鼠胚胎干细胞 (mESCs),我们揭示了一种机械机制,该机制基于胚胎内细胞团第二谱系分离成外胚层 (EPI) 细胞中的差异弹性 - 这将发育成胎儿 - 和原始内胚层 (PrE) - 将形成胚胎外结构,例如卵黄囊。值得注意的是,我们发现这些机械差异已经发生在启动过程中,而不仅仅是在细胞致力于分化之后。具体来说,我们表明,与任何其他报告的细胞类型相比,mESC 具有高弹性,并且 PrE 细胞比 EPI 引发的细胞更具弹性。使用仅弹性不同的两种细胞类型的模型,我们表明单独的差异弹性可以导致细胞类型之间的分离,这表明细胞的机械属性有助于分离过程。我们的研究结果表明差异弹性是胚胎形态发生过程中谱系分离的先前未知的机械贡献者。