Artificial reproduction of pikeperch (Sander lucioperca) is characterized by the unpredictability of latency time (LT) as well as the variability of egg quality depending on the time of hormonal stimulation. To predict latency time and thus enhance fertilization outcomes, the present study evaluated whether in vitro maturation techniques could be utilized to assess the status of oocyte maturation competence (OMC). Oocyte samples from nine pond-reared females originating from the Czech Republic (CZECH) and 12 intensively cultured females originating from river Körös (HAKI) were catheterized at the time of hormonal stimulation and cultured in L15 medium supplied with 100 ng/mL of DHP. The stage of final oocyte maturation (FOM) was monitored both in vivo and in vitro and egg quality features were evaluated. HAKI group was characterized by larger oocytes at the time of stimulation (934.4 ± 32.9 μm vs. 860.0 ± 22.7 μm), as well as higher embryo survival (77.7 ± 10.4% vs 53.5 ± 28.8%), suggesting that artificial reproduction was performed prematurely for CZECH group. Oocytes from HAKI fish displayed a higher share of maturing oocytes (41.4 ± 9.1% vs. 13.8 ± 17.6%) and in all samples germinal vesicle breakdown was noticed, while in the CZECH group it was so only in oocytes of one fish. With respect that all fish ovulated after the gonadoliberin stimulation, gonadotropin-regulated OMC is assumed. A correlation was found between the in vivo and in vitro LTs in the HAKI group only (r = 0.791, P = 0.002), indicating a certain autonomy of ovarian follicle of other parts of the physiological axis after completed OMC. The in vitro techniques show great promise for practical application in hatcheries to evaluate the aptness for hormonal stimulation on both individual and population levels.

梭鲈 ( Sander lucioperca ) 的人工繁殖的特点是潜伏期 (LT) 的不可预测性以及取决于激素刺激时间的卵子质量的可变性。为了预测潜伏时间并因此提高受精结果，本研究评估了体外成熟技术是否可用于评估卵母细胞成熟能力 (OMC) 的状态。来自捷克共和国 (CZECH) 的 9 名池塘饲养雌性和来自 Körös 河 (HAKI) 的 12 名密集培养雌性的卵母细胞样本在激素刺激时插入导管，并在提供 100 ng/mL DHP 的 L15 培养基中培养。最终卵母细胞成熟（FOM）的阶段在体内和评估了体外和卵子质量特征。HAKI 组的特点是在刺激时卵母细胞较大（934.4 ± 32.9 μm对860.0 ± 22.7 μm），以及更高的胚胎存活率（77.7 ± 10.4% vs 53.5 ± 28.8%），表明过早进行人工生殖对于捷克集团。来自 HAKI 鱼的卵母细胞显示出更高比例的成熟卵母细胞（41.4 ± 9.1%对13.8 ± 17.6%），并且在所有样本中都观察到了生泡破裂，而在捷克组中，仅在一条鱼的卵母细胞中如此。关于在促性腺激素刺激后排卵的所有鱼，假定促性腺激素调节 OMC。在体内和体外之间发现了相关性仅HAKI组LTs（r  =0.791，P  =0.002），说明OMC完成后生理轴其他部位的卵泡有一定的自主性。体外技术在孵化场的实际应用中显示出巨大的希望，以评估个体和群体水平的激素刺激适应性。