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Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio
Aquaculture ( IF 4.5 ) Pub Date : 2022-09-19 , DOI: 10.1016/j.aquaculture.2022.738854
Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motohiko Sano, Qiang Li

Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen that causes herpesviral haematopoietic necrosis (HVHN) disease to goldfish, Carassius auratus and gibel carp, C. auratus gibelio. In this study, the virus persistence or latency in apparently healthy farmed gibel carp and reactivation in vitro were investigated. All of the freshly dissected kidney tissues of 42 individuals of asymptomatic farmed gibel carp from six culture regions tested positive in PCR, indicating all fish were CyHV-2 carriers. The viral mRNA was detected in two of 42 fish: one fish expressed virus immediate-early (IE) gene ORF147 only, and another fish expressed IE gene ORF147, early (E) gene ORF35, and late (L) gene ORF72. Following the explant cultivation, the viral DNA copies in the kidney significantly increased in three fish including two individuals that expressed IE gene before explant cultivation. After explant cultivation, the expression of both the E and L genes was detected in the two fish in which active viral gene transcription was detected initially, and the infectious virus in those two fish was confirmed by an infection experiment. The study suggests CyHV-2 can establish persistent or latent infection in asymptomatic farmed gibel carp, and the different viral mRNA expression cascades were detected in different individuals. The persistent and latent virus can reactivate and be infectious, indicating that the apparently healthy gibel carp can be a source of infection.



中文翻译:

对明显健康的养殖鲫鱼 Carassius auratus gibelio 中 Cyprinid 疱疹病毒 2 潜伏期的调查

Cyprinid 疱疹病毒 2 (CyHV-2) 是一种高度传染性病原体,可导致金鱼、鲫鱼和鲫鱼、C. auratus gibelio发生疱疹病毒性造血坏死 (HVHN) 疾病. 在这项研究中,研究了明显健康的养殖河豚中病毒的持久性或潜伏期以及体外再激活。来自六个养殖区的 42 条无症状养殖河鲤个体的所有新鲜解剖的肾组织在 PCR 检测中均呈阳性,表明所有鱼类均为 CyHV-2 携带者。在 42 条鱼中的两条中检测到病毒 mRNA:一条鱼仅表达病毒即早 (IE) 基因 ORF147,另一条鱼表达 IE 基因 ORF147、早期 (E) 基因 ORF35 和晚期 (L) 基因 ORF72。外植体培养后,三条鱼肾脏中的病毒 DNA 拷贝显着增加,其中包括两个在外植体培养前表达 IE 基因的个体。外植体培养后,在最初检测到病毒基因转录活跃的两条鱼中检测到E和L基因的表达,并且通过感染实验证实了这两条鱼中的传染性病毒。该研究表明,CyHV-2 可以在无症状的养殖鲫鱼中建立持续性或潜伏性感染,并且在不同个体中检测到不同的病毒 mRNA 表达级联。持久性和潜伏性病毒可以重新激活并具有传染性,这表明表面上健康的鲫鱼可能是感染源。

更新日期:2022-09-22
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