Multimodal ion exchangers, containing ligands combining charged and hydrophobic moieties, exhibit enhanced separation compared to monomodal ion exchangers under low loading conditions. Commercial applications often require high protein loads and such conditions have not been investigated extensively, especially for multimodal anion exchangers. This work compares protein adsorption and separation by frontal chromatography for a commercial monomodal (Nuvia HP-Q) and two multimodal anion exchangers (Nuvia aPrime and Capto Adhere) using bovine serum albumin (BSA) monomer and dimer as a model.

中文翻译：

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用单峰和多峰阴离子交换色谱树脂吸附和分离蛋白质。第一部分. 多组分吸附性能和正面色谱

与低负载条件下的单峰离子交换剂相比，多峰离子交换剂含有结合了带电和疏水部分的配体，表现出增强的分离性。商业应用通常需要高蛋白质负载，并且此类条件尚未得到广泛研究，特别是对于多峰阴离子交换剂。这项工作使用牛血清白蛋白 (BSA) 单体和二聚体作为模型，比较了商业单峰 (Nuvia HP-Q) 和两种多峰阴离子交换剂 (Nuvia aPrime 和 Capto Adhere) 通过正面色谱法进行的蛋白质吸附和分离。