当前位置: X-MOL 学术Ocul. Surf. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Anti-inflammatory and wound healing potential of medicinal maggot excretions/secretions at the ocular surface
The Ocular Surface ( IF 6.4 ) Pub Date : 2022-09-18 , DOI: 10.1016/j.jtos.2022.09.003
Carolina Lema 1 , Hasna Baidouri 1 , Mingxia Sun 1 , Susanne Pohl 2 , Sharon Cookson 2 , Rachel Redfern 1 , Alison M McDermott 3
Affiliation  

Purpose

In the skin, Lucilia sericata maggot excretions/secretions (ES) accelerate wound healing and limit inflammation. This study aimed to determine whether ES have similar beneficial effects at the ocular surface.

Methods

Human corneal epithelial cells (HCEC) were cultured with ES and cell viability was determined by the MTT assay. Additionally, mRNA expression of growth factors, antimicrobial peptides (AMPs) and cytokines was assessed by qPCR. ES ability to modulate TLR-induced IL-6 and IL-8 expression was determined by qPCR and ELISA. ES potential to promote corneal healing was evaluated in vitro by a migration assay in HCEC, and in vivo using a mouse model.

Results

ES did not impair HCEC viability up to 25 μg/ml. Among the factors evaluated, only hBD-2 was upregulated (2.5-fold) by 1.5 μg/ml ES after 6 hrs (P = 0.04). In HCEC, ES reduced Poly I:C-induced IL-6 and IL-8 mRNA (P ≤ 0.001) and protein (P ≤ 0.0001) expression. A similar effect was observed with Flagellin (TLR5 agonist) but it was less robust for FSL-1 (TLR2/6 agonist) and Pam3CSK4 (TLR1/2 agonist). The greatest in vitro migration effect was observed with 6.2 μg/ml ES after 44 hrs where gap area compared to vehicle was 53.3 ± 3.7% vs. 72.6 ± 5.4% (P = 0.001). In the mouse model, the maximum healing effect was present with 1.5 μg/ml ES after 12 hrs with a wound area of 19.0 ± 2.7% vs. 60.1 ± 21.6% (P = 0.003) or 77% reduction of the wound area compared to the negative control.

Conclusions

ES significantly reduce in vitro TLR-induced production of inflammatory cytokines and promote corneal wound healing.



中文翻译:

眼表药用蛆排泄物/分泌物的抗炎和伤口愈合潜力

目的

在皮肤中,丝光绿蝇蛆的排泄物/分泌物 (ES) 可加速伤口愈合并限制炎症。本研究旨在确定 ES 是否对眼表具有类似的有益效果。

方法

用 ES 培养人角膜上皮细胞 (HCEC),并通过MTT 测定法测定细胞活力。此外,还通过 qPCR 评估了生长因子、抗菌肽 (AMP) 和细胞因子的 mRNA 表达。ES 调节 TLR 诱导的 IL-6 和 IL-8 表达的能力通过 qPCR 和 ELISA 确定。ES 促进角膜愈合的潜力通过 HCEC 中的迁移测定在体外使用小鼠模型在体内

结果

ES 不损害 HCEC 活力高达 25 μg/ml。在评估的因素中,只有 hBD-2 在 6 小时后被 1.5 μg/ml ES 上调(2.5 倍)(P  = 0.04)。在 HCEC 中,ES 降低了 Poly I:C 诱导的 IL-6 和 IL-8 mRNA ( P  ≤ 0.001) 和蛋白质 ( P  ≤ 0.0001) 的表达。用鞭毛蛋白(TLR5 激动剂)观察到类似的效果,但它对 FSL-1(TLR2/6 激动剂)和 Pam3CSK4(TLR1/2 激动剂)不太稳健。44 小时后使用 6.2 μg/ml ES 观察到最大的体外迁移效应,其中间隙面积与载体相比为 53.3 ± 3.7% 与 72.6 ± 5.4% (P = 0.001)。在小鼠模型中,12 小时后 1.5 μg/ml ES 的愈合效果最大,伤口面积为 19.0 ± 2.7% vs. 60.1 ± 21.6% ( P = 0.003) 或与阴性对照相比伤口面积减少 77%。

结论

ES 显着减少体外TLR 诱导的炎性细胞因子的产生并促进角膜伤口愈合。

更新日期:2022-09-18
down
wechat
bug