Here we present details of the calculation necessary to estimate the impact of avidity in a mathematical model of a bispecific antibody with two membrane-bound targets. The calculation is used to reproduce the results reported in Rhoden et al. (2016) and implemented in Kareva et al. (2018). We reproduce the impact of difference in relative concentration of the two targets on projections of free and bound concentrations of both targets and the antibody and highlight the applicability of this approach for supporting model informed decision making particularly in the early stages of drug discovery and development.

中文翻译：

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在具有两个膜结合靶标的双特异性抗体模型中计算亲和力影响的实用指南

在这里，我们介绍了在具有两个膜结合靶标的双特异性抗体的数学模型中估计亲和力影响所需的计算细节。该计算用于重现 Rhoden 等人报告的结果。（2016 年）并在 Kareva 等人中实施。（2018 年）。我们重现了两个靶标的相对浓度差异对靶标和抗体的游离和结合浓度预测的影响，并强调了这种方法在支持模型知情决策中的适用性，特别是在药物发现和开发的早期阶段。