当前位置: X-MOL 学术Plant Biotech. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A teosinte-derived allele of an HKT1 family sodium transporter improves salt tolerance in maize
Plant Biotechnology Journal ( IF 13.8 ) Pub Date : 2022-09-17 , DOI: 10.1111/pbi.13927
Ming Zhang 1, 2 , Yidan Li 3 , Xiaoyan Liang 1 , Minhui Lu 2 , Jinsheng Lai 2, 4 , Weibin Song 2, 4 , Caifu Jiang 1, 2
Affiliation  

The sodium cation (Na+) is the predominant cation with deleterious effects on crops in salt-affected agricultural areas. Salt tolerance of crop can be improved by increasing shoot Na+ exclusion. Therefore, it is crucial to identify and use genetic variants of various crops that promote shoot Na+ exclusion. Here, we show that a HKT1 family gene ZmNC3 (Zea mays L. Na+ Content 3; designated ZmHKT1;2) confers natural variability in shoot-Na+ accumulation and salt tolerance in maize. ZmHKT1;2 encodes a Na+-preferential transporter localized in the plasma membrane, which mediates shoot Na+ exclusion, likely by withdrawing Na+ from the root xylem flow. A naturally occurring nonsynonymous SNP (SNP947-G) increases the Na+ transport activity of ZmHKT1;2, promoting shoot Na+ exclusion and salt tolerance in maize. SNP947-G first occurred in the wild grass teosinte (at a allele frequency of 43%) and has become a minor allele in the maize population (allele frequency 6.1%), suggesting that SNP947-G is derived from teosinte and that the genomic region flanking SNP947 likely has undergone selection during domestication or post-domestication dispersal of maize. Moreover, we demonstrate that introgression of the SNP947-G ZmHKT1;2 allele into elite maize germplasms reduces shoot Na+ content by up to 80% and promotes salt tolerance. Taken together, ZmNC3/ZmHKT1;2 was identified as an important QTL promoting shoot Na+ exclusion, and its favourable allele provides an effective tool for developing salt-tolerant maize varieties.

中文翻译:

HKT1 家族钠转运体的类蜀黍衍生等位基因提高了玉米的耐盐性

钠阳离子 (Na + ) 是主要的阳离子,对受盐害农业地区的作物具有有害影响。作物的耐盐性可以通过增加地上部Na +排斥来提高。因此,识别和使用促进芽 Na +排斥的各种作物的遗传变异至关重要。在这里,我们表明 HKT1 家族基因ZmNC3Zea mays L. Na + Content 3;命名为ZmHKT1;2 )赋予玉米芽 Na +积累和耐盐性的自然变异性。ZmHKT1;2编码一个 Na +-位于质膜中的优先转运蛋白,介导芽 Na +排斥,可能是通过从根木质部流中撤回 Na + 。一个天然存在的非同义 SNP (SNP947-G) 增加了 ZmHKT1;2 的 Na +转运活性,促进了玉米芽的 Na +排除和耐盐性。SNP947-G 首先出现在野草类蜀黍中(等位基因频率为 43%),并已成为玉米种群中的次要等位基因(等位基因频率为 6.1%),表明 SNP947-G 来源于类蜀黍,基因组区域侧翼 SNP947 可能在玉米的驯化或驯化后传播过程中经历了选择。此外,我们证明了 SNP947-G ZmHKT1;2的基因渗入优良玉米种质中的等位基因可将地上部的 Na +含量降低高达 80%,并提高耐盐性。综上所述,ZmNC3/ZmHKT1;2被鉴定为促进地上部Na +排除的重要QTL ,其有利等位基因为开发耐盐玉米品种提供了有效工具。
更新日期:2022-09-17
down
wechat
bug