The chemical composition and antioxidant activity of extracts (POE) of Penicillium oxalate isolated from Ligusticum chuanxiong Hort have been investigated. However, the biological activity of POE is limited, and its antioxidant, stress resistance and DNA protection effects in vivo are unclear. The current study aims to explore the beneficial effects of POE on DNA damage protection in pBR322 plasmid and lymphocytes and stress resistance in Caenorhabditis elegans. The results showed that POE increased the survival rate of C. elegans under 35°C, UV and H2O2 stress, attenuated ROS and MDA accumulation, and enhanced the activity of some important enzymes (SOD, CTA, and GSH-PX). In addition, the POE-mediated stress resistance involved the upregulation of the expression of the sod-3, sod-5, gst-4, ctl-1, ctl-2, daf-16, hsp-16.1, hsp-16.2, and hsf-1 genes and acted dependently on daf-16 and hsf-1 rather than skn-1. Moreover, POE also reduced lipofuscin levels, but did not prolong the lifespan or damage the growth, reproduction and locomotion of C. elegans. Furthermore, POE showed a protective effect against DNA scission in the pBR322 plasmid and lymphocytes. These results suggested that P. oxalate extracts have significant anti-stress and DNA protection potential and could be potential drug candidates in the pharmaceutical field, thus greatly broadening the understanding of the biological effects of the endophytic fungus P. oxalate.

中文翻译：

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从川芎中分离出的内生真菌草酸青霉具有保护 DNA 损伤的潜力并增加秀丽隐杆线虫的抗逆性。

对川芎草酸青霉提取物(POE)的化学成分和抗氧化活性进行了研究。但POE的生物活性有限，其在体内的抗氧化、抗应激和DNA保护作用尚不清楚。本研究旨在探讨 POE 对 pBR322 质粒和淋巴细胞 DNA 损伤保护以及秀丽隐杆线虫抗逆性的有益作用。结果表明，POE提高了秀丽隐杆线虫在35℃、UV和H2O2胁迫下的存活率，减弱了ROS和MDA的积累，增强了一些重要酶（SOD、CTA和GSH-PX）的活性。此外，POE 介导的抗逆性涉及 sod-3、sod-5、gst-4、ctl-1、ctl-2、daf-16、hsp-16.1、hsp-16.2、和 hsf-1 基因，并且依赖于 daf-16 和 hsf-1 而不是 skn-1。此外，POE 还降低了脂褐质水平，但不会延长寿命或损害秀丽隐杆线虫的生长、繁殖和运动。此外，POE 对 pBR322 质粒和淋巴细胞中的 DNA 断裂具有保护作用。这些结果表明，草酸假单胞菌提取物具有显着的抗应激和DNA保护潜力，可能成为制药领域的潜在候选药物，从而大大拓宽了对内生真菌草酸假单胞菌生物学效应的认识。POE对pBR322质粒和淋巴细胞中的DNA断裂具有保护作用。这些结果表明，草酸假单胞菌提取物具有显着的抗应激和DNA保护潜力，可能成为制药领域的潜在候选药物，从而大大拓宽了对内生真菌草酸假单胞菌生物学效应的认识。POE对pBR322质粒和淋巴细胞中的DNA断裂具有保护作用。这些结果表明，草酸假单胞菌提取物具有显着的抗应激和DNA保护潜力，可能成为制药领域的潜在候选药物，从而大大拓宽了对内生真菌草酸假单胞菌生物学效应的认识。