Herbicides are the most commonly applied pesticides in Brazil, specifically those based on glyphosate, and are used for different crops, near the habitats of annual killifish. Annual killifish presents a short life cycle with generally restricted geographic distribution. In this context, we evaluated the effect of the Roundup Original© (65, 130 and 260 µg. L⁻¹ of glyphosate) herbicide on different development stages (adult-young and senile) of the annual killifish (Cynopoecilus sp.). We quantified the oxidative balance markers (superoxide dismutase, catalase, glutathione S-transferase, lipid peroxidation levels, and total proteins). We observed that the senile individuals presented 2-fold higher lipid peroxidation levels associated with the maintenance of superoxide dismutase and catalase activity levels even after exposure to the herbicide. However, senile subjects were negatively impacted by the exposure to formulations containing glyphosate, and this was related to a loss of glutathione S-transferase activity. Our research demonstrated that the established physiological markers and this species look promising for toxicology studies.

除草剂是巴西最常用的杀虫剂，特别是基于草甘膦的杀虫剂，用于一年生鳉鱼栖息地附近的不同作物。一年生鳉鱼生命周期短，地理分布普遍受限。在此背景下，我们评估了 Roundup Original©（65、130 和 260 µg.L ^-1草甘膦）除草剂对一年生鳉鱼（Cynopoecilussp。）。我们量化了氧化平衡标志物（超氧化物歧化酶、过氧化氢酶、谷胱甘肽 S-转移酶、脂质过氧化水平和总蛋白）。我们观察到，即使在接触除草剂后，老年个体的脂质过氧化水平也比维持超氧化物歧化酶和过氧化氢酶活性水平高出 2 倍。然而，老年受试者因接触含有草甘膦的配方而受到负面影响，这与谷胱甘肽 S-转移酶活性的丧失有关。我们的研究表明，已建立的生理标志物和该物种在毒理学研究中看起来很有希望。