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Lactic acid production from sugarcane field residue as renewable and economical bioresource by newly isolated Pediococcus pentosaceus HLV1
Biomass Conversion and Biorefinery ( IF 4 ) Pub Date : 2022-09-12 , DOI: 10.1007/s13399-022-03267-6
Harika Katepogu , Young Jung Wee , Suresh V. Chinni , Subash C. B. Gopinath , Asad Syed , Ali H. Bahkali , Abdallah M. Elgorban , Veeranjaneya Reddy Lebaka

Production of industrial solvents and chemicals from a renewable resource is quite appealing due to the dwindling fossil fuel reserves. It is essential to replace the utilization of plastic materials with biodegradable plastic materials like poly-lactic acid (PLA). In the current work, Pediococcus pentosaceus HLV1, a newly identified acetogenic strain, was used to produce LA from sugarcane field residue (SFR). One of the several lignocellulosic potential substrates that are readily available in tropical regions is SFR. SFR has a cellulose content of 40.3 3.5%, a hemicellulose content of 28.5 3.0%, and lignin content of 20.3 2.6%. Around 69% of the lignin and 73% of the hemicellulose were solubilized after the sequential dilute alkali and acid treatments. Cellulase was used to perform enzymatic saccharification of the pretreated substrate at a concentration of 10% (w/v), and produced 0.681of glucose per gram of pretreated biomass. 5 g/l of residual sugar and 61.5 g/l of LA were produced during the batch fermentation procedure. The actual fermentation procedure yielded a 1.12 g LA/g substrate used with a productivity of 0.86 g/L/h. HMF and furfural were reduced to 0.65 g/L and 0.15 g/L from 1.83 g/l to 0.91 g/l, respectively, after fermentation. In the polyphenols, the most inhibitory compounds vanillin and syringaldehyde were reduced to very low concentrations to 0.087 and 0.09 g/l from 0.284 to 1.536 g/l respectively. These compounds have not shown any inhibitory action on microbial growth and lactic acid production. The current study describes how SFR was sequentially acid pretreated and then enzymatically hydrolyzed to acquire the greatest amount of fermentable sugars, as well as how freshly isolated Pediococcus pentosaceus HLV1 was used it to produce lactic acid.



中文翻译:

新分离的戊糖片球菌 HLV1 从甘蔗田残渣中生产乳酸作为可再生和经济的生物资源

由于化石燃料储量不断减少,从可再生资源生产工业溶剂和化学品非常有吸引力。用聚乳酸 (PLA) 等可生物降解的塑料材料替代塑料材料的使用至关重要。在目前的工作中,戊糖片球菌HLV1 是一种新发现的产乙酸菌株,用于从甘蔗田残渣 (SFR) 中生产 LA。在热带地区很容易获得的几种木质纤维素潜在底物之一是 SFR。SFR的纤维素含量为40.3 3.5%,半纤维素含量为28.5 3.0%,木质素含量为20.3 2.6%。在连续的稀碱和酸处理后,大约 69% 的木质素和 73% 的半纤维素被溶解。纤维素酶用于以 10% (w/v) 的浓度对预处理底物进行酶促糖化,每克预处理生物质产生 0.681 葡萄糖。在分批发酵过程中产生了 5 g/l 的残糖和 61.5 g/l 的 LA。实际发酵过程产生 1.12 g LA/g 底物,使用的产率为 0.86 g/L/h。发酵后,HMF 和糠醛分别从 1.83 g/l 降低到 0.91 g/l 到 0.65 g/L 和 0.15 g/L。在多酚中,抑制性最强的化合物香草醛和丁香醛分别从 0.284 到 1.536 g/l 降低到非常低的浓度至 0.087 和 0.09 g/l。这些化合物对微生物生长和乳酸产生没有表现出任何抑制作用。目前的研究描述了 SFR 如何依次进行酸预处理,然后进行酶水解以获得最大量的可发酵糖,以及如何新鲜分离 这些化合物对微生物生长和乳酸产生没有表现出任何抑制作用。目前的研究描述了 SFR 如何依次进行酸预处理,然后进行酶水解以获得最大量的可发酵糖,以及如何新鲜分离 这些化合物对微生物生长和乳酸产生没有表现出任何抑制作用。目前的研究描述了 SFR 如何依次进行酸预处理,然后进行酶水解以获得最大量的可发酵糖,以及如何新鲜分离戊糖片球菌HLV1用于生产乳酸。

更新日期:2022-09-12
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