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High-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2022-09-08 , DOI: 10.1109/tnb.2022.3205057
Shuai Yuan , Yulin Zhang , Lang Nan , P.T. Lai , Tong Zhang , Philip W. T. Pong , Ho Cheung Shum

Microbes could be found almost everywhere around us and have significant impacts on our human society. The treatment of microorganisms has long been seen as a complex problem. Till now, most of the genetic and phenotypic information regarding rare species is buried in the bulk microbial colony due to a lack of efficient tools to screen live bacteria. Droplet microfluidics offers a powerful approach to address this problem. However, the interactions among bacteria and their living environment are entirely restricted by the water/oil interfaces in conventional water/oil single emulsion-based microfluidic systems. Here, we demonstrate an oil-mediated all-aqueous microfluidic workflow that can overcome this drawback. In contrast to the previous works, our all-aqueous culturing environment allows cell-cell and cell-environment interactions, thus facilitating the growth of bacteria. Fe 3 O 4 magnetic nanoparticles added into the alginate beads enables on-chip manipulation of the microcapsules. The core-shell structure separately encapsulates bacteria and magnetic particles in the core and shell to avoid contamination. We demonstrate the feasibility of this approach by single bacterium culturing in droplet-templated alginate beads. Finally, a new approach is proposed to degrade the alginate beads for post-treatment. This novel microfluidic workflow can create new opportunities for microbial applications, such as bacteria culturing and screening.

中文翻译:

用于单细菌培养分析的磁性纳米颗粒负载藻酸盐核壳珠的高通量生成、操作和降解

微生物几乎遍布我们周围的任何地方,并对人类社会产生重大影响。微生物的处理长期以来被视为一个复杂的问题。到目前为止,由于缺乏有效的活细菌筛选工具,有关稀有物种的大部分遗传和表型信息都被埋藏在大量微生物菌落中。液滴微流控技术提供了解决这一问题的有效方法。然而,在传统的基于水/油单乳液的微流体系统中,细菌与其生存环境之间的相互作用完全受到水/油界面的限制。在这里,我们展示了一种油介导的全水微流体工作流程,可以克服这个缺点。与以前的工作相比,我们的全水培养环境允许细胞-细胞和细胞-环境相互作用,从而促进细菌的生长。铁 添加到藻酸盐珠中的3 O 4磁性纳米粒子能够实现微胶囊的芯片上操作。核壳结构将细菌和磁性颗粒分别封装在核和壳内,避免污染。我们通过在液滴模板藻酸盐珠中培养单细菌来证明这种方法的可行性。最后,提出了一种新方法来降解藻酸盐珠以进行后处理。这种新颖的微流体工作流程可以为微生物应用创造新的机会,例如细菌培养和筛选。
更新日期:2022-09-08
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