当前位置: X-MOL 学术Gut Pathog. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Microbiota profile of new-onset celiac disease in children in Saudi Arabia
Gut Pathogens ( IF 4.2 ) Pub Date : 2022-09-08 , DOI: 10.1186/s13099-022-00493-1
Mohammad El Mouzan 1 , Abdulrahman Al-Hussaini 2, 3 , Gloria Serena 4, 5 , Asaad Assiri 1 , Ahmed Al Sarkhy 1 , Mohammad Al Mofarreh 6 , Mona Alasmi 1 , Alessio Fasano 4, 5, 7
Affiliation  

Intestinal dysbiosis has been reported to be associated with celiac disease (CeD) in Western populations but little is known in other populations who have different dietary lifestyle and genetic background. The purpose of this study was to determine whether a different microbiota profile is associated with CeD in children in Saudi Arabia. Forty children with CeD, 20 healthy controls, and 19 non-CeD controls were enrolled. The median age at diagnosis was 10.3, 11.3 and 10.6 years in children with CeD, fecal, and mucosal control groups, respectively. Significant differences in microbial composition between children with CeD and controls both at fecal and mucosal level were identified. Fecal samples were more diverse and richer in bacteria as compared with mucosal samples. Proteobacteria were more abundant in duodenal mucosal samples and Firmicutes and Bacteroides were more abundant in stools. The abundance of many taxa was significantly different between children with CeD and non-CeD controls. In mucosal samples, Bifidobacterium angulatum (unadjusted p = 0.006) and Roseburia intestinalis (unadjusted p = 0.031) were examples of most significantly increased species in children with CeD and non-CeD controls, respectively. In fecal samples, there were 169 bacterial species with significantly different abundance between children with CeD and non- CeD controls. To our knowledge, this is the first report on the microbial profile in a non-Western population of children with new onset CeD. The fact that mucosal and fecal samples were collected from newly diagnosed children with CeD on normal gluten-containing diet suggests strong association between the identified bacteria and CeD. The identification of many unreported bacterial species significantly associated with CeD, indicates the need for further studies from different populations to expand our understanding of the role of bacteria in the pathogenesis of CeD, hopefully leading to the discovery of new adjuvant treatment options.

中文翻译:

沙特阿拉伯儿童新发乳糜泻的微生物群特征

据报道,在西方人群中,肠道菌群失调与乳糜泻(CeD)有关,但在其他饮食生活方式和遗传背景不同的人群中却知之甚少。本研究的目的是确定不同的微生物群谱是否与沙特阿拉伯儿童的 CeD 相关。招募了 40 名患有 CeD 的儿童、20 名健康对照和 19 名非 CeD 对照。CeD、粪便和黏膜对照组儿童的中位诊断年龄分别为 10.3、11.3 和 10.6 岁。确定了 CeD 患儿与对照组在粪便和粘膜水平上的微生物组成存在显着差异。与粘膜样本相比,粪便样本更多样化且细菌含量更高。十二指肠黏膜样本中的变形菌更丰富,粪便中的厚壁菌门和拟杆菌门更丰富。许多分类群的丰度在 CeD 和非 CeD 对照儿童之间存在显着差异。在黏膜样本中,角双歧杆菌(未经调整的 p = 0.006)和罗斯氏菌(未经调整的 p = 0.031)分别是 CeD 和非 CeD 对照儿童中物种最显着增加的例子。在粪便样本中,有 169 种细菌种类,在 CeD 和非 CeD 对照儿童之间存在显着不同的丰度。据我们所知,这是第一份关于新发 CeD 的非西方儿童人群微生物谱的报告。黏膜和粪便样本是从新诊断的 CeD 正常含麸质饮食儿童身上采集的,这一事实表明已鉴定的细菌与 CeD 之间存在很强的关联。许多未报告的与 CeD 显着相关的细菌种类的鉴定表明,需要对不同人群进行进一步的研究,以扩大我们对细菌在 CeD 发病机制中的作用的理解,并有望发现新的辅助治疗方案。
更新日期:2022-09-09
down
wechat
bug