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Antigen-Down PEC Immunosensor for CYFRA21-1 Detection Based on Photocurrent Polarity Switching Strategy
Analytical Chemistry ( IF 7.4 ) Pub Date : 2022-08-31 , DOI: 10.1021/acs.analchem.2c01478
Jinhuan Zhang 1, 2 , Xiaodong Xue 3 , Yizhen Du 3 , Jinxiu Zhao 1 , Hongmin Ma 1, 2 , Xiang Ren 1, 2 , Qin Wei 1, 2 , Huangxian Ju 1, 2
Affiliation  

In this work, an antigen-down photoelectrochemical (PEC) immunosensor based on a signal polarity switching strategy for the detection of cytokeratin 19 fragment 21-1 (CYFRA21-1) was proposed. 3,4,9,10-Perylene tetracarboxylic acid (PTCA) is a conjugated organic dye containing five benzene nuclei, which has excellent film-forming and optical properties. PTCA sensitized by SnS2 can further improve the basal signal and the stability of the PEC immunosensor. Moreover, avidin-functionalized CuInS2 as a signal probe can convert the basal anodic photocurrent to a cathodic photocurrent. Therefore, the PEC sensor realized the photocurrent polarity conversion before and after labeling. With avidin-functionalized CuInS2, the polarity of the photocurrent was changed once CYFRA21-1 was detected. Therefore, the PEC immunosensor owns high sensitivity. The linear range of the immunosensor for the detection of CYFRA21-1 is 0.00001–500 ng·mL–1, and the detection limit is 3.5 fg·mL–1. The PEC immunosensor has good stability, high selectivity, and good repeatability. This work may provide a new way for the detection of CYFRA21-1 and other proteins.

中文翻译:

基于光电流极性切换策略的 CYFRA21-1 检测的抗原下调 PEC 免疫传感器

在这项工作中,提出了一种基于信号极性切换策略的抗原下调光电化学 (PEC) 免疫传感器,用于检测细胞角蛋白 19 片段 21-1 (CYFRA21-1)。3,4,9,10-苝四羧酸(PTCA)是一种含有五个苯核的共轭有机染料,具有优异的成膜性和光学性能。SnS 2敏化的PTCA可以进一步提高PEC免疫传感器的基础信号和稳定性。此外,亲和素功能化的CuInS 2作为信号探针可以将基础阳极光电流转换为阴极光电流。因此,PEC传感器实现了标记前后的光电流极性转换。具有抗生物素蛋白功能化的 CuInS 2,一旦检测到 CYFRA21-1,光电流的极性就会改变。因此,PEC免疫传感器具有较高的灵敏度。该免疫传感器检测CYFRA21-1的线性范围为0.00001-500 ng·mL –1,检测限为3.5 fg·mL –1。PEC免疫传感器稳定性好、选择性高、重复性好。该工作可能为CYFRA21-1等蛋白的检测提供新的途径。
更新日期:2022-08-31
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