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Towards mapping of Helminthosporium leaf blight/Pyrenophora leaf spot resistance genes/QTLs in oats
Cereal Research Communications ( IF 1.6 ) Pub Date : 2022-08-30 , DOI: 10.1007/s42976-022-00306-w
Harshavardan J. Hilli , Rahul Kapoor , Ashlesha Singla , Priti Sharma , Puja Srivastava

The oat crop has been neglected due to its use in limitation as a forage crop rather than a staple food in most of the countries throughout the world. Also it is more prone to foliar diseases, especially Helminthosporium leaf blight (HLB, syn Pyrenophora leaf spot) disease in the Punjab, especially Ludhiana region. It emerged as a new disease that can infect over 70% of the field resulting in up to forage yield losses of 50 per cent throughout. Hence, this study was conducted to evaluate the 167 germplasm lines for disease severity and its infection based on 1–9 scale through artificial inoculation of fungal spores. These lines were classified under four different clusters based upon infection with majority falling under cluster 2 and cluster 3 with infection of more than 50%. Lines ranged from being highly susceptible (more than 75% infection) to moderately resistant (22% infection). These contrasting lines were used in disease progression studies and also in hybridization programme to develop populations for future mapping studies. The contrasting lines were analysed for polymorphism using 220 SSR markers, of which 47 markers were polymorphic. Also the mapping for HLB resistance genes/QTLs is being carried out under the current ongoing research project, which will be the first research to map the resistance genes/QTLs in the oats throughout India and the world. Identified lines also can be used in future breeding programme to develop resistant cultivars.



中文翻译:

燕麦中蠕虫叶枯病/Pyrenophora叶斑病抗性基因/QTL的定位

燕麦作物由于在世界上大多数国家被限制用作饲料作物而不是主食而被忽视。此外,它更容易发生叶面病害,尤其是旁遮普省,尤其是卢迪亚纳地区的蠕孢叶枯病(HLB,syn Pyrenophora 叶斑病)病害。它作为一种新疾病出现,可以感染超过 70% 的田地,导致整个草料产量损失高达 50%。因此,本研究通过人工接种真菌孢子,以 1-9 级评估 167 个种质系的疾病严重程度及其感染情况。这些品系根据感染分为四个不同的集群,其中大多数属于集群 2 和集群 3,感染率超过 50%。品系的范围从高度易感(超过 75% 的感染)到中等耐药(22% 的感染)。这些对比线被用于疾病进展研究和杂交计划,以开发用于未来绘图研究的人群。使用 220 个 SSR 标记分析对比品系的多态性,其中 47 个标记是多态性的。此外,HLB 抗性基因/QTL 的绘图正在当前正在进行的研究项目下进行,这将是第一个在印度和世界范围内绘制燕麦抗性基因/QTL 的研究。鉴定的品系也可用于未来的育种计划以开发抗性品种。这些对比线被用于疾病进展研究和杂交计划,以开发用于未来绘图研究的人群。使用 220 个 SSR 标记分析对比品系的多态性,其中 47 个标记是多态性的。此外,HLB 抗性基因/QTL 的绘图正在当前正在进行的研究项目下进行,这将是第一个在印度和世界范围内绘制燕麦抗性基因/QTL 的研究。鉴定的品系也可用于未来的育种计划以开发抗性品种。这些对比线被用于疾病进展研究和杂交计划,以开发用于未来绘图研究的人群。使用 220 个 SSR 标记分析对比品系的多态性,其中 47 个标记是多态性的。此外,HLB 抗性基因/QTL 的绘图正在当前正在进行的研究项目下进行,这将是第一个在印度和世界范围内绘制燕麦抗性基因/QTL 的研究。鉴定的品系也可用于未来的育种计划以开发抗性品种。这将是第一个在印度和世界范围内绘制燕麦抗性基因/QTL 的研究。鉴定的品系也可用于未来的育种计划以开发抗性品种。这将是第一个在印度和世界范围内绘制燕麦抗性基因/QTL 的研究。鉴定的品系也可用于未来的育种计划以开发抗性品种。

更新日期:2022-09-01
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