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Transcriptional Memory in Taraxacum mongolicum in Response to Long-Term Different Grazing Intensities
Plants ( IF 4.658 ) Pub Date : 2022-08-30 , DOI: 10.3390/plants11172251
Yalin Wang 1, 2 , Wenyan Zhu 3 , Fei Ren 4 , Na Zhao 3 , Shixiao Xu 3 , Ping Sun 1, 4
Affiliation  

Grazing, as an important land use method in grassland, has a significant impact on the morphological and physiological traits of plants. However, little is known about how the molecular mechanism of plant responds to different grazing intensities. Here, we investigated the response of Taraxacum mongolicum to light grazing and heavy grazing intensities in comparison with a non-grazing control. Using de novo transcriptome assembly, T. mongolicum leaves were compared for the expression of the different genes under different grazing intensities in natural grassland. In total, 194,253 transcripts were de novo assembled and comprised in nine leaf tissues. Among them, 11,134 and 9058 genes were differentially expressed in light grazing and heavy grazing grassland separately, with 5867 genes that were identified as co-expression genes in two grazing treatments. The Nr, SwissProt, String, GO, KEGG, and COG analyses by BLASTx searches were performed to determine and further understand the biological functions of those differentially expressed genes (DEGs). Analysis of the expression patterns of 10 DEGs by quantitative real-time RT-PCR (qRT-PCR) confirmed the accuracy of the RNA-Seq results. Based on a comparative transcriptome analysis, the most significant transcriptomic changes that were observed under grazing intensity were related to plant hormone and signal transduction pathways, carbohydrate and secondary metabolism, and photosynthesis. In addition, heavy grazing resulted in a stronger transcriptomic response compared with light grazing through increasing the of the secondary metabolism- and photosynthesis-related genes. These changes in key pathways and related genes suggest that they may synergistically respond to grazing to increase the resilience and stress tolerance of T. mongolicum. Our findings provide important clues for improving grassland use and protection and understanding the molecular mechanisms of plant response to grazing.

中文翻译:

蒲公英对长期不同放牧强度的转录记忆

放牧作为草地重要的土地利用方式,对植物的形态和生理性状具有重要影响。然而,关于植物的分子机制如何响应不同的放牧强度知之甚少。在这里,我们研究了蒲公英与非放牧对照相比对轻放牧和重放牧强度的反应。使用从头转录组组装,T. mongolicum比较天然草地不同放牧强度下不同基因的表达情况。总共有 194,253 个转录本从头组装并包含在九个叶组织中。其中,11134和9058个基因分别在轻度放牧和重度放牧草地中差异表达,5867个基因在两个放牧处理中被鉴定为共表达基因。通过 BLASTx 搜索进行 Nr、SwissProt、String、GO、KEGG 和 COG 分析以确定和进一步了解这些差异表达基因 (DEG) 的生物学功能。通过定量实时 RT-PCR (qRT-PCR) 分析 10 个 DEGs 的表达模式证实了 RNA-Seq 结果的准确性。基于比较转录组分析,在放牧强度下观察到的最显着的转录组变化与植物激素和信号转导途径、碳水化合物和次生代谢以及光合作用有关。此外,通过增加次生代谢和光合作用相关基因的数量,与轻度放牧相比,重度放牧导致了更强的转录组反应。关键途径和相关基因的这些变化表明,它们可能协同响应放牧,以增加放牧的恢复力和抗压能力。与轻度放牧相比,重度放牧通过增加次生代谢和光合作用相关基因的数量而导致更强的转录组反应。关键途径和相关基因的这些变化表明,它们可能协同响应放牧,以增加放牧的恢复力和抗压能力。与轻度放牧相比,重度放牧通过增加次生代谢和光合作用相关基因的数量而导致更强的转录组反应。关键途径和相关基因的这些变化表明,它们可能协同响应放牧,以增加放牧的恢复力和抗压能力。蒙古毛茛。我们的研究结果为改善草地利用和保护以及了解植物对放牧反应的分子机制提供了重要线索。
更新日期:2022-08-30
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