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Preventive Effect and Mechanism of Anthocyanins from Aronia Melanocarpa Elliot on Hepatic Fibrosis Through TGF-β/Smad Signaling Pathway
Cell Biochemistry and Biophysics ( IF 2.6 ) Pub Date : 2022-08-30 , DOI: 10.1007/s12013-022-01079-z
Ruobing Hao 1 , Jun Gao 2 , Hongwei Liu 1 , Chenjuan Zhang 1 , Jinpeng Huang 1 , Jungang Fan 2 , Jie Wei 1
Affiliation  

In order to explore the effect and mechanism of Aornia mealnocarpa Elliot anthocyanins (AMA) at the cellular level on hepatic fibrosis (HF), molecular docking, RT-PCR and Western Blotting were used to explore the molecular mechanism and the effects of different doses AMA on HSC-T6 cells by TGF-β1 induction. The results showed that the binding energy of anthocyanins on TGF-β1 (PDB ID: 3KFD) was in the range of −9.5 to 8.6 kcal/mol, with good low energy parameters and binding positions. AMA could effectively inhibit the expressions of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin (TSB), and improved the expressions of total protein (TP) and albumin (ALB). RT-PCR and Western bloting results showed that AMA could inhibited the secretion of inflammatory cytokines IL-1, IL-6, TNF-α and COX-2, and inhibit the expression of TGF-β1, P-Smad2, α-SMA and Collagen I in TGF-β /Smad signaling pathway. This study revealed the AMA’s inhibition effects and mechanism of malignant biological behavior of HSC-T6 cells, in order to provide theoretical basis for the prevention and treatment of HF by Aronia melanocarpa Elliot.



中文翻译:

野樱莓花青素通过TGF-β/Smad信号通路对肝纤维化的预防作用及机制

为探讨牛蒡的作用及机制采用细胞水平艾略特花青素(AMA)对肝纤维化(HF)、分子对接、RT-PCR和Western Blotting等方法探讨不同剂量AMA对TGF-β1诱导HSC-T6细胞的分子机制和影响。结果表明,花青素对TGF-β1(PDB ID:3KFD)的结合能为-9.5~8.6 kcal/mol,具有良好的低能参数和结合位点。AMA能有效抑制天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)和血清总胆红素(TSB)的表达,改善总蛋白(TP)和白蛋白(ALB)的表达。RT-PCR和Western blotting结果显示,AMA可以抑制炎性细胞因子IL-1、IL-6、TNF-α和COX-2的分泌,抑制TGF-β1、P-Smad2、TGF-β/Smad 信号通路中的 α-SMA 和胶原蛋白 I。本研究揭示了AMA对HSC-T6细胞恶性生物学行为的抑制作用及机制,旨在为HF的防治提供理论依据。Aronia melanocarpa Elliot。

更新日期:2022-08-30
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