Assessing mammalian gene function in vivo has traditionally relied on manipulation of the mouse genome in embryonic stem cells or perizygotic embryos. These approaches are time-consuming and require extensive breeding when simultaneous mutations in multiple genes is desired. The aim of this study is to introduce a rapid in vivo multiplexed editing (RIME) method and provide proof of concept of this system.

中文翻译：

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成年小鼠肝脏的快速体内多重编辑 (RIME)

评估哺乳动物体内基因功能传统上依赖于对胚胎干细胞或合子胚胎中的小鼠基因组的操作。当需要多个基因同时突变时，这些方法非常耗时并且需要广泛的育种。本研究的目的是介绍一种快速体内多重编辑（RIME）方法并提供该系统的概念证明。