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Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress
Journal of Molecular Histology ( IF 3.2 ) Pub Date : 2022-08-29 , DOI: 10.1007/s10735-022-10099-1
Renqun Mao 1 , Dalian Du 2 , Xiaodi Zhu 1 , Wenqing Li 1
Affiliation  

Functionalizing biomaterial substrates with biological signals shows promise in regulating cell behaviors through mimicking cellular microenvironment. Calcium phosphate (CaP) coating is an excellent carrier for immobilizing biological molecules due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. In this study, we reported the adhesion, the viability and proliferation behaviors after oxidative stress injury of Schwann cells RSC96 on CaP immobilized with the Velvet Antler Peptide (VAP) isolated from velvet antler through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing VAP. This approach provided well retention of functional molecules up to 28 days, and supported the adhesion and proliferation of RSC96 after oxidative stress injury without cytotoxicity. The simple and reproducible method of coprecipitation suggests that CaP is an ideal carrier to functionalize materials with biological molecules for peripheral nerve repair-related applications.



中文翻译:

鹿茸多肽结合磷酸钙涂层保护周围神经细胞免受氧化应激

用生物信号功能化生物材料底物有望通过模拟细胞微环境来调节细胞行为。磷酸钙(CaP)涂层由于其无毒、良好的生物相容性、生物降解性和对大量分子的良好亲和力,是一种用于固定生物分子的优良载体。在这项研究中,我们报道了雪旺细胞 RSC96 在氧化应激损伤后在改良 Dulbecco 磷酸盐缓冲盐水 (DPBS) 中通过共沉淀法从鹿茸中分离出来的鹿茸肽 (VAP) 固定化 CaP 后的粘附、活力和增殖行为。含有 VAP。这种方法可以很好地保留功能分子长达 28 天,并支持 RSC96 在氧化应激损伤后无细胞毒性的粘附和增殖。这种简单且可重现的共沉淀方法表明,CaP 是一种理想的载体,可以将具有生物分子的材料功能化,用于周围神经修复相关的应用。

更新日期:2022-08-30
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