A controlled release immunosensor for the rapid detection of lung cancer tumor marker CYFRA21–1 was designed based on a personal glucose meter (PGM). Here, polyethyleneimine modified mesoporous silica nanoparticles (MSN-PEI) were used as nanocontainers to load glucose, antibody labeled gold nanoparticles (Au NPs-Ab) were used as molecular gates to block the pores of MSN-PEI. When CYFRA21–1 antigen was added into the solution, the CYFRA21–1 antigen could specifically recognize and combine with its antibody to form complex. Thereafter, the bio-gate opened and the glucose released from the pores which could be detected on the personal glucose meter. Under the optimized conditions, the proposed PGM-based immunosensor exhibited a wide linear response to CYFRA21–1 ranging from 1.3 ng/mL to 160 ng/mL, and its detection limit is 0.79 ng/mL, it opened up a new way for the biological analysis of CYFRA21–1 and other biomarkers.

基于个人血糖仪（PGM）设计了一种用于快速检测肺癌肿瘤标志物CYFRA21-1的控释免疫传感器。在这里，聚乙烯亚胺修饰的介孔二氧化硅纳米粒子（MSN-PEI）被用作纳米容器来装载葡萄糖，抗体标记的金纳米粒子（Au NPs-Ab）被用作分子门来阻断MSN-PEI的孔隙。当溶液中加入CYFRA21-1抗原后，CYFRA21-1抗原可特异性识别并与其抗体结合形成复合物。此后，生物门打开，葡萄糖从毛孔中释放出来，可以在个人血糖仪上检测到。在优化条件下，所提出的基于 PGM 的免疫传感器对 CYFRA21-1 的线性响应范围为 1.3 ng/mL 至 160 ng/mL，检测限为 0.79 ng/mL，