The common carbamate insecticide aldicarb is considered one of the most acutely toxic pesticides. Herein, rational design was used to synthesize two haptens with spacers of different carbon chain lengths. The haptens were then used to immunize mice. The antibodies obtained were evaluated systematically, and a colloidal gold immunochromatographic strip was developed based on an anti-aldicarb monoclonal antibody. The 50% inhibition concentration and linear range of anti-aldicarb monoclonal antibody immunized with Hapten 1 were 0.432 ng/mL and 0.106–1.757 ng/mL, respectively. The cross-reactivities for analogs of aldicarb were all <1%. The limit of detection of the colloidal gold immunochromatographic strip was 30 μg/kg, and the average recoveries of aldicarb ranged from 80.4 to 110.5% in spiked samples. In the analysis of spiked samples, the test strip could accurately identify positive samples detected by the instrumental method in the GB 23200.112-2018 standard but produced some false positives for negative samples. This assay provides a rapid and accurate preliminary screening method for the determination of aldicarb in agricultural products and environments.

常见的氨基甲酸酯杀虫剂涕灭威被认为是毒性最强的杀虫剂之一。在此，合理设计用于合成两种具有不同碳链长度的间隔物的半抗原。然后将半抗原用于免疫小鼠。对获得的抗体进行系统评价，以抗涕灭威单克隆抗体为基础研制胶体金免疫层析条。Hapten 1免疫抗涕灭威单克隆抗体的50%抑制浓度和线性范围分别为0.432 ng/mL和0.106-1.757 ng/mL。涕灭威类似物的交叉反应性均<1%。胶体金免疫层析条的检出限为30 μg/kg，加标样品中涕灭威的平均回收率为80.4%~110.5%。在加标样品的分析中，该试纸可以准确识别GB 23200.112-2018标准中仪器方法检测出的阳性样品，但对阴性样品产生了一些假阳性。该测定为农产品和环境中涕灭威的测定提供了一种快速准确的初步筛选方法。