Purpose

Osteogrow, an osteoinductive device containing recombinant human Bone Morphogenetic Protein 6 (rhBMP6) in autologous blood coagulum, is a novel therapeutic solution for bone regeneration. This study aimed to evaluate different commercially available calcium phosphate synthetic ceramic particles as a compression-resistant matrix (CRM) added to Osteogrow implants to enhance their biomechanical properties.

Methods

Osteogrow implants with the addition of Vitoss, ChronOs, BAM, and Dongbo ceramics (Osteogrow-C, where C stands for ceramics) were evaluated in the rodent subcutaneous ectopic bone formation assay. Osteogrow-C device was prepared as follows: rhBMP6 was added to blood, and blood was mixed with ceramics and left to coagulate. Osteogrow-C was implanted subcutaneously in the axillary region of Sprague–Dawley rats and the outcome was analyzed 21 days following implantation using microCT, histology, morphometric analyses, and immunohistochemistry.

Results

Osteogrow-C implants with all tested ceramic particles induced the formation of the bone-ceramic structure containing cortical bone, the bone between the particles, and bone at the ceramic surfaces. The amount of newly formed bone was significant in all experimental groups; however, the highest bone volume was measured in Osteogrow-C implants with highly porous Vitoss ceramics. The trabecular number was highest in Osteogrow-C implants with Vitoss and ChronOs ceramics while trabeculae were thicker in implants containing BAM and Dongbo ceramics. The immunological response and inflammation were comparable among ceramic particles evaluated in this study.

Conclusion

Osteogrow-C bone regenerative device was effective with a broad range of commercially available synthetic ceramics providing a promising therapeutic solution for the regeneration of long bone fracture nonunion, large segmental defects, and spinal fusion surgeries.

中文翻译：

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用含有 rhBMP6 的自体血凝块配制的合成陶瓷产品用于诱导骨形成的比较

目的

Osteogrow 是一种在自体凝血中含有重组人骨形态发生蛋白 6 (rhBMP6) 的骨诱导装置，是一种新型的骨再生治疗方案。本研究旨在评估不同的市售磷酸钙合成陶瓷颗粒作为抗压基质 (CRM) 添加到 Osteogrow 植入物以增强其生物力学性能。

方法

在啮齿动物皮下异位骨形成试验中评估了添加 Vitoss、ChronOs、BAM 和 Dongbo 陶瓷（Osteogrow-C，其中 C 代表陶瓷）的 Osteogrow 植入物。Osteogrow-C装置的制备如下：将rhBMP6加入血液中，将血液与陶瓷混合并静置凝固。在 Sprague-Dawley 大鼠的腋窝区域皮下植入 Osteogrow-C，并在植入后 21 天使用 microCT、组织学、形态学分析和免疫组织化学分析结果。

结果

带有所有测试陶瓷颗粒的 Osteogrow-C 植入物诱导了包含皮质骨、颗粒之间的骨和陶瓷表面处的骨的骨陶瓷结构的形成。在所有实验组中，新形成的骨量均显着；然而，在采用高多孔 Vitoss 陶瓷的 Osteogrow-C 种植体中测量到最高的骨量。含有 Vitoss 和 ChronOs 陶瓷的 Osteogrow-C 种植体的骨小梁数量最多，而含有 BAM 和 Dongbo 陶瓷的种植体的骨小梁数量较多。在本研究中评估的陶瓷颗粒之间的免疫反应和炎症具有可比性。

结论

Osteogrow-C 骨再生装置与广泛的市售合成陶瓷有效，为长骨骨折不愈合、大节段缺损和脊柱融合手术的再生提供了有希望的治疗解决方案。