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Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations
Electrophoresis ( IF 2.9 ) Pub Date : 2022-08-20 , DOI: 10.1002/elps.202200130
Veronika Šolínová 1 , Petr Tůma 2 , Maria Butnariu 1, 3 , Václav Kašička 1 , Dušan Koval 1
Affiliation  

We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonate (AMPS), that is, poly(AM-co-AMPS) or PAMAMPS. Coatings were formed by the in-capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10−9 m2 V−1 s−1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS-treated capillaries, we observed (i) a negligible dependence on pH in the 2–10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter-EOF mode.

中文翻译:

用于优化毛细管电泳分离的具有可调电渗流的共价阴离子共聚物涂层

我们提出了一种在阴极方向精细调节电渗流 (EOF) 速度的方法,用于优化毛细管电泳中的分离。为此,我们使用丙烯酰胺 (AM) 和 2-acrylamido-2-methyl-1-propanesulfonate (AMPS) 的共价连接共聚物对分离熔融石英毛细管进行表面改性,即聚 (AM- co - AMPS ) ) 或 PAMAMP。涂层是由中性 AM 和阴离子 AMPS 单体以不同比例预混合的混合物的毛细管内聚合形成的,以控制共聚物的电荷密度。EOF 迁移率在 0 到 ∼40 × 10 -9  m 2 V -1 s -1范围内变化PAMAMPS 涂层的间隔范围为 0 至 60 mol.% 的带电 AMPS 单体。对于 PAMAMPS 处理的毛细血管中的 EOF,我们观察到(i)在 2-10 间隔内对 pH 值的依赖性可以忽略不计,(ii)背景电解质(BGEs)在其成分功能方面的微小差异和(iii)其标准随着增加 BGE 的离子强度。通过在反 EOF 模式下改善两种异构阴离子分析物的分离,即单糖磷酸盐和 helquat 对映体,证明了对可变阴极 EOF 的兴趣。
更新日期:2022-08-20
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